20.500.12556/DKUM-73577 Študija proizvodnosti hidrolize saharoze z encimom vezanim na odpadna steklena vlakna magistrsko delo Study of reaction rate of sucrose hydrolysis with enzyme bounded to the waste glass fibre V raziskavi smo kot nosilec za imobilizacijo encima invertaze uporabili odpadna steklena vlakna. Encim smo kovalentno vezali na modificirani nosilec. Učinkovitost imobilizacije smo testirali na reakciji hidrolize saharoze. Vse eksperimente smo izvajali v laboratorijskem šaržnem reaktorju. Preučevali smo vpliv pH (4,5, 5, 5,5 in 6) in reakcijske temperature (20, 25, 30 in 35) °C na presnovo reakcije. Učinkovitost reakcije smo spremljali in-situ z merjenjem koncentracije saharoze, glukoze in fruktoze z uporabo FTIR sonde. Na koncu smo preverili vpliv pH (4,5, 5, 5,5 in 6) imobilizacije na presnovo pri predhodno določenih optimalnih pogojih reakcije. Dosegli smo visoko učinkovitost imobilizacije. Določili smo optimalen pH reakcijske mešanice in temperaturo reakcije. Nadalje smo ob večkratni zaporedni uporabi katalizatorja določili aktivnost invertaze, imobilizirane na steklena vlakna. Raziskavo smo nadgradili s kinetično študijo reakcije. Iz enačbe encimske kinetike smo določili konstante proizvodnosti ter z uporabo Arrhenius-ove zveze aktivacijsko energijo in pred-eksponentni faktor. In this study we have used waste glass fibers as a carrier for immobilization of enzyme invertase. The enzyme was bound to a modified carrier by a covalent bond. .Efficiency of immobilization was tested on sucrose hydrolysis reaction. All experiments were conducted in laboratory batch reactor. Effect of pH (4,5, 5, 5,5 in 6) and reaction temperature (20, 25, 30 in 35) °C on reaction yield was explored. Reaction effectiveness was monitored in-situ by measuring sucrose, fructose and glucose concentration using FTIR probe. In the end, we have explored the effect of pH (4,5, 5, 5,5 in 6) of immobilization at determined optimal conditions on reaction yield. We have achieved high effectiveness of immobilization. We determined optimal pH value of reaction mixture and reaction temperature. Furthermore, the activity of invertase immobilized to fiberglass was determined by multiple consecutive use of enzyme. The study was advanced by kinetic study of reaction. We have determined the productivity constants with enzyme kinetic equation. We have also determined activation energy and pre-exponential factor from Arrhenius plot. steklena vlakna invertaza hidroliza saharoze imobilizacija fiberglass invertase sucrose hydrolysis immobilization true true false [A. Marin] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2019-05-16 22:16:14 2019-05-31 14:13:52 2022-08-02 06:01:51 2019-08-06 03:30:30 2019 0 Maribor 0 Maribor XII, 73 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 664.16.094.941:577.15(043.2) 22418966 URN:SI:UM:DK:PB78GJCI MAG_Marin_Aljaz_2019.pdf MAG_Marin_Aljaz_2019.pdf 1 8BC36ED69BEC47CD8FF013E1750F3198 91728c1f3cad25dc85ab4a41ff3c0623cd44bfa5c87d9ab20c9c1d502f3a09b6 b7548972-7c10-11eb-bb7a-00155d0001ca 20.500.12556/dkum/3eef6151-2f11-4642-9612-5f2a934b7e43 https://dk.um.si/Dokument.php?lang=slv&id=134204 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0