20.500.12556/DKUM-73835 Vpliv v gojišče dodanega meta-topolina na rast in razraščanje marelice (Prunus armeniaca L.) v tkivnih kulturah Effect of meta-Topolin added to the medium on growth and shoot formation of apricot (Prunus armeniaca L.) in tissue culture Na Fakulteti za kmetijstvo in biosistemske vede smo v letu 2019 proučevali vpliv dodanega meta-topolina v rastno gojišče na rast in razraščanje marelice v tkivnih kulturah. Za rastlinski material smo uporabili lokalni genotip marelice, poznan pod imenom Pišečka marelica, ki je že bila iniciirana v tkivne kulture. Marelico smo razmnoževali na osnovnem gojišču McCown Woody Plant, ki smo mu dodali različne koncentracije meta-topolina (mT) ter 1-naftilocetne kisline (NAA). Pripravili smo kontrolno gojišče (G0), ki ni vsebovalo rastnih hromonov, gojišče G1, ki je vseboval 0,5 mg/l mT in 0,01 mg/l NAA ter gojišče G2, ki je vsebovalo 1,0 mg/l mT in 0,01 mg/l NAA. Opazovali smo povečanje prirasta mase, število poganjkov ter delež vitrifikacije pri rastlinah na posameznem gojišču. Ugotovili smo, da se je najbolje izkazalo gojišče G2, na katerem smo zabeležili povprečni 19,5 faktorski prirast oz. 0,372 g sveže mase, 4,5 poganjka na rastlino, noben poganjek ni bil vitrificiran. Pri gojišču G1 smo opazili 17,9 faktorski prirast oz. 0,326 g povprečne mase, 4,2 poganjka ter en vitrificiran poganjek, medtem ko so bile vrednosti pri kontrolnem gojišču značilno nižje – 3,8 faktorski prirast oz. 0,071 g mase, 1,22 poganjka in trije primeri vitrifikacije. S statistično obdelavo podatkov smo ugotovili tudi korelacijo med prirastom mase rastlin ter številom poganjkov (r = 0,85) in korelacijo med faktorskim povečanjem koncentracije hormonov v gojišču in prirastom mase rastlin (r = 0,63) ter števila poganjkov (r = 0,61). In 2019, an experiment was conducted at the Faculty of Agriculture and Life Sciences of the University of Maribor, where the effect of meta-Topolin added to the medium on growth and branching of apricots was studied. The apricot local varietal material Pišečka that was used in this experiment, was already introduced to the tissue culture. Increase of plantlet weight, number of shoots and rate of vitrification in the media with different concentrations of plant growth hormones were observed. The McCown Woody Plant was used as the growth medium, to which different concentrations of meta-topolin (mT) and 1-naphthaleneacetic acid (NAA) were added. The growth media were divided into 3 groups: the control medium G0, which did not contain growth regulators, the medium G1, to which 0.5 mg/l mT and 0.01 mg/l NAA were added, and the medium G2 which contained 1.0 mg/l mT in 0.01 mg/l NAA. The medium G2 produced the best results with a factor of 19.5 or 0.372 g plantlet weight increase, 4.5 shoots per plantlet and zero vitrification rate. In G1 medium a factor of 17.9 or 0.326 g weight increase, 4.2 shoots per plantlet and one case of vitrification were documented. As for the control medium, a significantly lower success was obtained. The values of the control medium were 3,8 factorial increase or 0.071 g plantlet weight increase, 1.22 shoots per plantlet and no vitrification noted in any of plantlets. A positive correlation between plantlet weight increase and number of shoots was determined (r = 0.85). Futhermore, correlations between the factorial increase of hormones in the medium and weight increase (r = 0.63), along with number of shoots per plantlet (r = 0.61) were perceived. marelica Prunus armeniaca L. tkivne kulture prirast poganjki apricot Prunus armeniaca L. tissue culture plant weight shoots true true false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2019-06-19 21:26:21 2019-06-28 10:07:42 2022-08-02 06:23:16 2019-07-26 11:54:24 2019 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:9NAJOTED VS_Hlebs_Lana_2019.pdf VS_Hlebs_Lana_2019.pdf 1 83A6D3F963A8177B4EBEFA4497BA116C 76f55851b91f6a26d3a0a77639d88e501ae4fc7b42caa540a244aa915168f301 c19d3095-7c10-11eb-bb7a-00155d0001ca 20.500.12556/dkum/8f8c9839-fbfd-4441-889d-63b49da86f7b https://dk.um.si/Dokument.php?lang=slv&id=134783 Fakulteta za kmetijstvo in biosistemske vede 0 0 0