20.500.12556/DKUM-74173 Vpliv osvetljenosti smreke (Picea abies) na vsebnost vitamina C v smrekovih vršičkih The Effect of Insolation on the Vitamin C Content in Spruce (Picea abies) Tips Cilji raziskave so bili preučiti vpliv osvetljenosti in starosti dreves na koncentracijo vitamina C v smrekovih vršičkih ter raziskati vsebnost vitamina C v sirupu, pripravljenem iz smrekovih vršičkov. Vršičke smo nabrali na Sv. Treh kraljih na Pohorju. V izvlečkih vršičkov in sirupa smo koncentracijo skupnega vitamina C določili po redukciji dehidroaskorbinske kisline v askorbinsko kislino z dodatkom ditiotreitola. Vsebnost vitamina C smo določali s tekočinsko kromatografijo visoke ločljivosti (HPLC). Povprečna vsebnost vitamina C v vršičkih s sončne lege je znašala 1087 mg/100 g suhe snovi, v vršičkih s senčne lege pa 916 mg/100 g suhe snovi. V sirupu je bila povprečna vsebnost vitamina C (11,6 mg/100 g sveže snovi) bistveno nižja kot v vršičkih. S statistično analizo rezultatov smo ugotovili, da je korelacija med vsebnostjo vitamina C v smrekovih vršičkih in sirupu statistično značilna. Vršički s sončne strani imajo značilno višjo vsebnost vitamina C kot vršički s senčne strani. Korelacija med vsebnostjo vitamina C v smrekovih vršičkih in obsegom debla (s tem tudi s starostjo) smreke ni statistično značilna. The purpose of the presented study was to investigate the effects of insolation and tree age on the vitamin C content in spruce tips. Additionally, we measured the content of vitamin C in spruce tip syrup. Spruce tips were collected at the location of Sv. Trije kralji on Pohorje, NE Slovenia. The concentration of total vitamin C in the extracts of the spruce tips and the syrup was determined after the reduction of dehydroascorbic acid to ascorbic acid with the addition of dithiothreitol. The concentration of vitamin C in spruce tips and syrup was measured by high performance liquid chromatography (HPLC). The average value of vitamin C in spruce tips that were exposed to sunlight was 1087 mg/100 g of dry weight and in shaded spruce tips 916 mg/100 g of dry weight. The average vitamin C content in syrup (11,6 mg/100 g of fresh weight) was considerably lower when compared to the average value obtained for the spruce tips. Statistical analysis of the results showed that the correlation between vitamin C content in spruce tips and syrup is statistically significant. Insolated spruce tips showed a significantly higher level of vitamin C than shaded tips. The correlation between vitamin C content in spruce tips and trunk girth (age) was not statistically significant. smreka (Picea abies smrekovi vršički sirup vitamin C osvetljenost spruce (Picea abies) spruce tips syrup vitamin C insolation true true false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2019-08-14 10:45:31 2019-08-27 09:52:06 2022-08-09 08:22:00 2019-08-28 03:24:02 2019 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:CBST5JT9 UN_Celcar_Spela_2019.pdf UN_Celcar_Spela_2019.pdf 1 89527774108E779E30A123383AFF4066 ccc43f1bbb7d029c4d72c8a37a5ad25da4ec729d26b59e4aefbd366d38fda256 d169975f-7c10-11eb-bb7a-00155d0001ca 20.500.12556/dkum/4ab74c56-c2f3-4e84-8b0a-2e55f8d67e19 https://dk.um.si/Dokument.php?lang=slv&id=135774 Fakulteta za kmetijstvo in biosistemske vede 0 0 0