20.500.12556/DKUM-74802 Antimikrobna učinkovitost Punica granatum L. na Escherichia coli diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje Antimicrobial efficiency of Punica granatum L. on Escherichia coli Namen diplomske naloge je pridobiti učinkovine iz različnih delov Punica granatum L. in proučiti njihov inhibitorni učinek na rast mikrobnih celic Escherichia coli ter določiti vsebnost totalnih fenolov in vitamina C v vzorcih. S klasično vodno ekstrakcijo in Soxhletovo ekstrakcijo z etanolom smo pridobili učinkovine iz svežih in liofiliziranih semen, membrane in eksokarpa Punica granatum L. Proučili smo inhibitorni učinek ekstraktov na rast mikrobne kulture E. coli z disk difuzijsko metodo ter določili vsebnost totalnih fenolov in vitamina C v vzorcih. Ugotovili smo, da se v celotnem sadežu Punica granatum L. nahajajo totalni fenoli, vitamin C in antimkrobne spojine, ki zavirajo rast bakterijske kulture E. coli. Koncentracije naštetih spojin so najvišje v membrani in eksokarpu Punica granatum L., saj smo v njihovih ekstraktih določili najvišjo vsebnost totalnih fenolov in vitamina C in omenjena dela Punica granatum L. izkazujeta tudi največji inhibitorni učinek na rast E. coli. Najmanj totalnih fenolov in vitamina C se nahaja v ekstraktih semen. The purpose of this work was to obtain bioactive substances from different parts of pomegranate (Punica granatum L.) and to study their inhibitory properties on the growth of Escherichia coli microbial cells and the content of total phenols and vitamin C. With the use of classical aqueous extraction and Soxhlet extraction with ethanol we have extracted bioactive substances from fresh and lyophilized pomegranate seeds, membranes and exocarp. The extracts were then tested for their inhibitory properties on the growth of Escherichia coli microbial culture by disk diffusion method. They were also tested for their total phenol content and vitamin C content. The results indicated that one whole pomegranate fruit contains total phenols, vitamin C and antimicrobial compounds that inhibit the growth of Escherichia coli bacterial culture. The concentration of these compounds is highest in the pomegranate membrane and exocarp, and the lowest in pomegranate seeds. Punica granatum L. antimikrobne lastnosti totalni fenoli vitamin C inhibicija bakterije sekundarni metaboliti pomegranate antimicrobial properties total phenols vitamin C inhibition bacteria secondary metabolites true true false [A. Peklar] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2019-09-07 12:59:21 2019-10-10 13:10:25 2022-08-09 09:34:24 2019-10-19 03:13:12 2019 0 Maribor 0 Maribor IX, 29 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 [604.4:615.33]:582.772.1(043.2) 22676758 URN:SI:UM:DK:XEAIBLFT VS_Peklar_Anze_2019.pdf VS_Peklar_Anze_2019.pdf 1 8D83BE02BE1A886471966388E6FF2675 baebbe2180424b297746c10c1f4525f58242726a6738b8b30958dd839d097066 fc2bb319-7c10-11eb-bb7a-00155d0001ca 20.500.12556/dkum/d15a0ef8-1eed-42c4-b3dd-8824ddf6ed4f https://dk.um.si/Dokument.php?lang=slv&id=138094 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0