20.500.12556/DKUM-75860 Utjecaj eikosanoida na kontrakciju i relaksaciju glatkih mišića respiratornog puta Effect of eicosanoids on the contraction and relaxation of airway smooth muscles Eikosanoidi su metaboliti arahidonske kiseline. Između njih prostaglandini i leukotrieni imaju najvažnije biološke učinke i uloge u organizmu. Jedna od tih uloga je kontrakcija/relaksacija glatkih mišića respiratornog sustava. Tako je dereguliranjem produkcije eikosanoida moguća bronhokonstrikcija kod astme. U ovom radu usredotočujemo se na ovisnost sile glatkih mišića različitih tkiva iz različitih subjekata, čovjeka i životinja o različitim vrstama eikosanoida. Opisat ćemo mehanizme nastanka eikosanoida, receptore preko kojih oni djeluju te njihovu funkciju. Analizirali smo mjerenja kontrakcije i relaksacije glatkih mišića dišnih putova pod utjecajem različitih eikosanoida. Među ljudskim tkivima analizirane su sile razvijene u glatkim mišićima bronha, malih dišnih puteva te plućnog parenhima. Kod životinjskog modela uzeti su uzorci tkiva glatkih mišića s dušnika miša te zamorca. Digitalizacijom mjerenja obradili smo podatke iz različitih znanstvenih članaka. Koristeći računalni program Origin 8.5 s alatom Digitizer, digitalizirali smo ovisnosti dišnih putova glatkih mišića različitih organizama o koncentracijama eikosanoida i drugih kontraktilnih agonista te odredili parametre tih ovisnosti prilagođavanjem različitih odabranih matematičkih funkcija (različiti oblici Hill funkcije). Napravili smo usporedbu parametara između različitih organizama i između mjerenja različitih autora. Nadalje, usporedili smo jačine eikosanoidne kontrakcije s drugim fiziološkim kontraktilnim agonistima i na kraju objasnili je li moguće prenijeti podatke izmjerene na životinjama u fizikalno-matematičke modele, koji simuliraju procese kod ljudi. Eicosanoids are the metabolites of arachidonic acid. Among them prostaglandins and leukotrienes have the most important biological activities and roles in the human organism. One of these roles is related to the contraction/relaxation of smooth muscles, especially in airways. Their dysregulation in production may lead to bronchoconstriction in asthma. Here we focus on the relationship of the developed force in airway smooth muscle cells taken from different tissues and from different subjects, human and animal, with respect to different eicosanoids. First, the mechanisms of the production of eicosanoids, their action through receptors and their function is described. The main goal was to analyse the measurements of the developed force in airway smooth muscles with respect to different eicosanoids, either contractile or relaxing. In case of human tissues, we analysed the developed force in smooth muscles of bronchi, small airways and the pulmonary parenchyma. In case of animals, the analysis was performed for the smooth muscles of the mouse and guinea pig tracheas. For this purpose we first digitized measured dependencies of forces versus eicosanoid concentrations with the Digitizer tool within the computer program Origin 8.5. Then we fitted these dependencies with different forms of Hill functions and determined the parameter values within these functions. Further, we compared these parameters between different organisms and between measurements of different authors. Based on this information, the strength of contractile and relaxation eicosanoids was evaluated. Finnaly, by comparing the parameter values obtained from human with those from animals, we determined, whether the results differed from each other and whether the data obtained from animals could be transferred to physical-mathematical models that simulate processes in humans. prostaglandini leukotrieni arahidonska kiselina bronhokonstrikcija digitalizacija. prostaglandins leukotrienes arachidonic acid bronchoconstriction digitization. true false false [K. Blažetić] Hrvaški jezik Angleški jezik Magistrsko delo/naloga 2020-02-20 20:46:57 2020-06-04 13:55:42 2022-08-09 11:50:38 2020-06-05 03:11:55 2020 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577:616.2:519.87(043.2) 18062595 URN:SI:UM:DK:8KGYFCEM MAG_Blazetic_Karlo_2020.pdf MAG_Blazetic_Karlo_2020.pdf 1 AD1405120FB3F7DA4CB75957056B82CC b6d61e6dbdff2d2bc72c2c2b387de315e78e724e48e9af1e547a84db266b4d2d 3a94b030-7c11-11eb-bb7a-00155d0001ca 20.500.12556/dkum/54fd3653-4d54-45ce-9635-7f7a312bfd39 https://dk.um.si/Dokument.php?lang=slv&id=140745 Fakulteta za zdravstvene vede 0 0 0