20.500.12556/DKUM-76148 Sočasno določanje ferulne, kavne in p-kumarne kisline v rastlinskih vzorcih s tekočinsko kromatografijo magistrsko delo Simultaneous determination of ferulic, caffeic and p-coumaric acid in plant samples with liquid chromatography Določali smo vsebnosti ferulne kisline, kavne kisline in p-kumarne kisline v vzorcu rdečega grozdja, v trgovsko dosegljivem rožmarinovem ekstraktu in v trgovsko dosegljivem ekstraktu zelene kave. Preiskovane kisline smo določali s tekočinsko kromatografijo visoke ločljivosti z UV zaznavo. Za ločevanje spojin smo uporabili izokratsko elucijo, kjer smo kot mobilno fazo uporabili mešanico metanola, vode in ocetne kisline v volumskem razmerju 15:80:5. Spojine v ekstraktih smo potrdili s primerjavo retenzijskih časov standardov, kvantifikacijo pa smo izvedli na podlagi umeritvenih krivulj. Linearnost metode je bila za vse preiskovane kisline potrjena v koncentracijskem območju 1,0–100,0 mg/L. Vzorce smo pripravljali z različnimi postopki ekstrakcij (ekstrakcija tekoče-tekoče, ekstrakcija trdno tekoče z etilacetatom v Soxhletovem aparatu in s pomočjo ultrazvoka, ekstrakcija po kislinski hidrolizi in ekstrakcija po alkalni hidrolizi vzorcev). Najvišje vsebnosti preiskovanih spojin v rdečem grozdju so bile določene z ekstrakcijo po kislinski hidrolizi vzorcev kot tudi po ultrazvočni ekstrakciji. V vzorcu rožmarinovega ekstrakta in ekstrakta zelene kave preiskovanih kislin nismo zaznali. The contents of ferulic acid, caffeic acid and p-coumaric acid in samples of red grapes, commercially available rosemary extract and in commercially available green coffee extract were determined. The tested acids were determined by high performance liquid chromatography using UV-detection. An isocratic elution was used for the separation of the compounds. A mixture of methanol, water, and acetic acid in volume ratio of 15: 80: 5 was used as the mobile phase. The compounds in the extracts were confirmed by comparing the retention times of standards, and the quantification was performed on the basis of calibration curves. The linearity of the method for all acids was confirmed in the concentration range of 1.0-100.0 mg/L. The samples were prepared by various extraction processes (liquid-liquid or solid-liquid extraction with ethyl acetate in the Soxhlet apparatus or in an ultrasound bath, extraction with acid hydrolysis, and extraction with alkaline hydrolysis of the samples). The highest contents of the investigated compounds in red grapes were determined by extraction with acid hydrolysis of the samples as well as after ultrasonic extraction. The investigated acids were not detected in samples of rosemary and green coffee extract. Validacija ekstrakcija ferulna kislina kavna kislina p-kumarna kislina rastlinski vzorci tekočinska kromatografija. Validation extraction ferulic acid caffeic acid p-coumaric acid plants samples liquid chromatography. true true false [M. Krempl] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2020-04-20 11:03:47 2020-06-12 10:48:31 2022-08-09 12:10:03 2020-06-18 03:10:22 2020 0 Maribor 0 Maribor IX, 46 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.544.5:547.587(043.2) 19692291 URN:SI:UM:DK:YYRAGJI1 MAG_Krempl_Mateja_2020.pdf MAG_Krempl_Mateja_2020.pdf 1 B89ED34C482FD3C3ED087A7E5B470BDF 724fed341c9240b980444b006e29971f063c99cc3566e0ab85ff4d34b166aae8 54557e78-7c11-11eb-bb7a-00155d0001ca 20.500.12556/dkum/a1f2aa8c-866d-46c8-ae23-cc6daf8ce88b https://dk.um.si/Dokument.php?lang=slv&id=142397 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0