20.500.12556/DKUM-76670 Razporeditev genotipov človeških papilomavirusov v različnih oblikah kožnih bradavic Distribution of human papillomavirus types in different types of cutaneous warts Poznavanje razporeditve vzročnih genotipov HPV v najpogostejših kliničnih tipih kožnih bradavic (KB) je izjemno pomembno za razvoj preventivnih cepiv proti kožnim genotipom HPV. V tej raziskavi so bili na osnovi ocenjenih virusnih bremen opredeljeni vzročni genotipi HPV v 203 sveže zamrznjenih tkivnih vzorcih histopatološko potrjenih KB - 185 navadnih (NB) in 18 plantarnih bradavicah (PB), ki so bile odvzete skupno 138 imunsko-kompetentnim bolnikom. Vsi tkivni vzorci so bili s tipsko specifično kvantitativno verižno reakcijo s polimerazo v realnem času (PCR) testirani na najpogostejše Alphapapillomaviruse (Alpha-PV) ki so potencialni povzročitelji KB (HPV-2/-27/-57) in Mu-PV (HPV-1/-63/-204) ter s konvencionalnim širokospektralnim PCR-jem za opredeljevanje prisotnosti 23 nizkorizičnih HPV. Deoksiribonukleinska kislina HPV je bila identificirana v 176/185 (95 %) NB in v vseh PB. Na podlagi ocenjenih virusnih bremen večine opredeljenih genotipov HPV in določene mejne vrednosti pri eni virusni kopiji/celico so bili v tej raziskavi kot potencialni povzročitelji kožnih sprememb (vzročni genotipi HPV) opredeljeni HPV-2/-27/-57 (v približno treh četrtinah NB in v dveh tretjinah PB) ter HPV-1 (v 4 % NB in v eni tretjini PB). Čeprav so bile sočasne okužbe z več genotipi HPV identificirane v približno eni tretjini NB in PB, so bile potencialne vzročne so-okužbe opredeljene v samo 2 % NB, medtem ko jih v vzorcih PB nismo zaznali. Naše ugotovitve nakazujejo na nekoliko različno razporeditev potencialnih vzročnih genotipov HPV-2/-27-/57 in HPV-1 v NB in PB, s predominanco genotipa HPV1 za plantarno lokacijo, kar potrjuje potrebo po vključitvi virusom podobnih delcev genotipov HPV-2/-27-/57/-1 v bodoče cepivo proti kožnim genotipom HPV. Knowledge of distribution of causative human papillomavirus (HPV) types in the most prevalent clinical types of cutaneous warts is of the utmost importance in the development of prophylactic cutaneous HPV vaccines. In this study, the potentially causative HPV types were determined based on estimated viral loads (VLs) of specific HPV types, in 203 fresh-frozen tissue specimens of histologically confirmed cutaneous warts - 185 common warts (CWs) and 18 plantar warts (PWs), collected from the total of 138 immunocompetent patients. All tissues were tested using the type-specific quantitative real-time polymerase chain reactions (PCRs) for the most common wart-associated Alphapapillomavirus (Alpha-PV) (HPV2/27/57) and Mu-PV types (HPV1/63/204). The presence of 23 additional low-risk HPVs was evaluated using a conventional wide-spectrum PCR. HPV DNA was detected in 176/185 (95.1%) CWs and in all PWs. Based on estimated VLs of the majority of detected HPV types and a robust cutoff of one viral copy/cell established in this study, HPV2/27/57 types were determined as potentially causative HPV types in three quarters of CWs and in two thirds of PWs, whereas HPV1 was assigned as potentially causative HPV type in 4% of CWs and in one third of PWs. While mixed HPV infections were detected in one-third of CWs and PWs, potentially causative HPV co-infections were ascertained in only 2 % of CWs and in none of the PWs. Our findings suggest a fairly different distribution of potentially causative HPV2/27/57 types and HPV1 type in CWs and PWs with predominance of causative HPV1 type for plantar location, and reinforce the inclusion of virus-like particles of HPV2/27/57/1 types in the future cutaneous HPV vaccine. človeški papilomavirusi HPV genotip rod kožne bradavice navadne bradavice plantarne bradavice virusno breme citopatogeni učinek histopatologija PCR genotipizacija inkluzije human papillomaviruses HPV type genus cutaneous warts common warts plantar warts viral load cytopathic effect histopathology PCR genotypisation inclusion true true false Slovenski jezik Angleški jezik Doktorsko delo/naloga 2020-06-19 01:57:10 2021-02-05 14:06:05 2022-08-09 13:49:54 2021-02-06 03:18:42 2020 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 50415619 URN:SI:UM:DK:U610UBPX DOK_Breznik_Vesna_2021.pdf DOK_Breznik_Vesna_2021.pdf 1 628B5F701D485EB968D0EBE8BE9AE3BF f8e52b5598dfcfb1846a1342ce6d603627e29d48776044f31edb42fb058191b1 1885737f-7c12-11eb-bb7a-00155d0001ca 20.500.12556/dkum/7e514d65-8ccf-48de-b25f-d92e2fe01765 https://dk.um.si/Dokument.php?lang=slv&id=148628 Medicinska fakulteta 0 0 0