20.500.12556/DKUM-77099 Vpliv v gojišče dodanih avksinov in tretiranja s temo na koreninjenje marelice v tkivni kulturi The effect of auxins added to culture medium and dark treatment on apricot-tree root formation in plant tissue culture. Leta 2019 smo na Fakulteti za kmetijstvo in biosistemske vede raziskali vpliv avksinov IBA in NAA ter vpliv teme na rast in razvoj korenin pri marelici (Prunus armeniaca L.) v in vitro razmerah. Uporabili smo poganjke marelice, ki so že rastli v tkivni kulturi. Kontrolno gojišče G0 ni vsebovalo avksinov. Gojišče G1 je vsebovalo 1 mg/l IBA, G2 2 mg/l IBA, G3 4 mg/l IBA, G4 0,2 mg/l NAA in G5 1 mg/l NAA. Za vsak tip gojišča smo pripravili 60 kozarčkov. Poganjke smo pustili na gojišču za koreninjenje tri tedne. Polovico kozarčkov (30) smo prekrili z alufolijo in jih po sedmih dneh odkrili (tema). Na kontrolnem gojišču je bil delež ukoreninjenja 9,1-odstoten. Najvišji delež (100 %) ukoreninjenja je bil na gojišču G1, ki je bilo izpostavljeno temi. Najnižji delež ukoreninjenja (11,5 %) pa je bil na gojišču G5 na svetlobi. Koncentracija avksina je statistično značilno vplivala na število korenin. Pri dodanem avksinu IBA je bilo povprečno število korenin 3,260, v temi pa 2,544. Pri dodanem avksinu NAA je bilo povprečno število korenin 0,452, v temi pa 1,095. Ugotovili smo tudi, da dodatek avksina NAA statistično značilno vpliva na pojavljanje kalusa, dodatek avksina IBA pa ne. In 2019, an experiment was conducted at the Faculty of Agriculture and Life Sciences to study the effect of IBA and NAA auxins and dark treatment on the growth and development of roots in apricot plantlets (Prunus armeniaca L.) under in vitro conditions. Apricot shoots that were already growing in plant tissue culture were used. The control medium G0 did not contain any auxins. The medium G1 contained 1 mg/L of IBA, medium G2 2 mg/L of IBA, medium G3 3 4 mg/L of IBA, medium G4 0.2 mg/L of NAA and medium G5 1 mg/L of NAA. For every medium type, 60 jars were prepared. The shoots were left on the rooting medium for three weeks. Half of the jars (30) were covered in aluminium foil and the uncovered after 7 days (dark treatment). The rooting percentage on the control medium was 9.1%. The highest rooting percentage (100%) was found on medium G1 which was subjected to dark treatment. The lowest rooting percentage was found on medium G5 in daylight. The auxin concentration had a statistically significant impact on the number of roots. When auxin IBA was added. The average number of roots was 3.260, and 2.544 in the dark. When auxin NAA was added, the average number of roots was 0.452 and 1.095 in the dark. It was found that the addition of auxin NAA had s statistically significant impact on callus formation, whereas the addition of auxin IBA did not. marelica mikropropagacija in vitro koreninjenje Apricot micropropagation in vitro rooting true true false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2020-08-17 11:03:36 2020-09-02 14:09:47 2022-08-09 14:21:43 2020-09-04 03:14:09 2020 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:WV6A9FNI UN_Horvat_Katja_2020.pdf UN_Horvat_Katja_2020.pdf 1 976028EBAC90380F2FAAC19887D9FC71 cab20de78f3cdd892654bc0e21e524f93d1841e2c98c62ecad003dd5469b20b8 72d8af88-7c11-11eb-bb7a-00155d0001ca 20.500.12556/dkum/7eb4f53a-85d2-41b3-acdf-5f2f6712ea36 https://dk.um.si/Dokument.php?lang=slv&id=144150 Fakulteta za kmetijstvo in biosistemske vede 0 0 0