20.500.12556/DKUM-77493 Stabilnost cinkovega oksida v gelskih strukturah in njegovo antibakterijsko delovanje diplomsko delo univerzitetnega študijskega programa I. stopnje Stability of zinc oxide in gel structures and its antibacterial activity Namen diplomske naloge je bila sinteza antimikrobnih gelskih struktur, ki bi se lahko uporabljale v medicini za oskrbo in celjenje ran. Uporabili smo polisaharidne materiale, saj so ne toksični, biorazgradljivi in v naravi prisotni v velikih količinah. Med sintezo smo gelskim strukturam dodali nanodelce cinkovega oksida n(ZnO), saj delujejo antimikrobno. Gelske strukture smo ustrezno karakterizirali z infrardečo spektroskopijo s Fourierjevo transformacijo (FTIR), z vrstičnim elektronskim mikroskopom (ESEM), merjenjem kontaktnih kotov, vezave tekočine ter antimikrobne teste s pomočjo disk difuzne metode. Gelske strukture z različnimi deleži ZnO smo izpostavili bakterijam Escherichia coli in Staphylococcus aureus z namenom določitve njihovega antibakterijskega delovanja. Ugotovili smo, da imajo liofilizirane gelske strukture visoko stopnjo poroznosti, so hidrofilne, dobro vežejo puferske tekočine ter so v primeru kovalentnega vezanega n(ZnO) tudi antimikrobne. Za antibakterijsko delovanje je pomembna interakcija med karboksimetil skupino in Zn, ki stabilizira ujetje nanodelcev v gelski strukturi. The goal of the diploma was the synthesis of antimicrobial gel structures, which could be used in medicine for healing wounds. We used polysaccharidic materials, because they aren't toxic, biologically disposable and available in nature in big amounts. We added nano particles of zinc oxide n(ZnO) to the gel structures during the synthesis, because they have antimicrobial effects. We characterised the gel structures suitably with infrared spectroscopy by using the Fourier transformation (FTIR), with a scanning electron microscope (ESEM), by measuring the contact angle, the fluid's inflection and antimicrobial tests using the disc diffusion method. To determine whether or not they are antimicrobially viable, we exposed gel structures with different amounts of ZnO to the bacteria Escherichia coli and Staphylococcus aureus. The results showed that lyophilised gel structures contain a high level of porosity, are hydrophile, join with buffer fluids very well and are antimicrobial if bonded covalently with n(ZnO). It's important for the carboxymethyl groups to interact with Zn to ensure the antimicrobial activity, which stabilizes the caught nanoparticles inside of the gel structure. cinkov oksid gelske strukture antibakterijsko delovanje FTIR ESEM zinc oxide hydrogels antibacterial activity FTIR ESEM true true false [S. Karlovšek] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2020-09-01 17:30:38 2020-10-08 14:02:07 2022-08-09 15:38:10 2020-10-15 03:21:20 2020 0 Maribor 0 Maribor XI, 35 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 604.4:615.281.9(043.2) 32289283 URN:SI:UM:DK:YC1142S7 UN_Karlovsek_Sara_2020.pdf UN_Karlovsek_Sara_2020.pdf 1 03A0105AA54200864C3812A9F2A54483 3e8860a45dda6c0797a5d31e5ca210af9d96aeb0950934fa8bffdb81f75ce4f0 d9f3c9d7-7c11-11eb-bb7a-00155d0001ca 20.500.12556/dkum/19986081-94da-48fd-895d-46319c1e1cf8 https://dk.um.si/Dokument.php?lang=slv&id=146026 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0