20.500.12556/DKUM-77837 Antioksidativna aktivnost nekaterih vrst in medvrstnih križancev bezga v modelni celični liniji TLT z metodo uporabe barvila DCFH-DA. Antioxidant Potential of Some Species and Interspecific Hybrids of Elderberries Studied in a TLT Model Cell Line Based on DCFH-DA Staining Antioksidativne snovi pozitivno vplivajo na celice telesa, saj izboljšujejo njihov fitnes in tako pripomorejo k boljšemu zdravju ljudi. Namen te raziskave je bil dokumentirati antioksidativne lastnosti ekstraktov bezgovih jagod. V raziskavo je bilo vključenih 9 različnih genotipov bezga (vrsti Sambucus javanica in S. nigra ter 7 medvrstnih križancev). Antioksidativno aktivnost ekstraktov bezgov smo izmerili s pomočjo uporabe barvila DCFH-DA (2´,7´-diklorofluorescin-diacetat) v pogojih in vitro na modelni celični makrofagni liniji TLT. Na različne načine smo ovrednotili rezultate fluorescence, saj ni določenega enotnega načina za izračun znotrajceličnega antioksidativnega potenciala v primerjavi s pozitivno kontrolo ali standardnimi antioksidanti. Ugotovili smo, da imajo proučevani genotipi bezgov različni znotrajcelični antioksidativni potencial. Najvišji znotrajcelični antioksidativni potencial ima vrsta Sambucus nigra (NI), najnižjega pa medvrstni križanec (JA × NI) × MIQ. Rezultati bi lahko bili zelo koristni za nadaljnjo selekcijo in vzgojo križancev bezga. Antioxidant substances have positive effects on body cells by improving their fitness and hence they contribute to better human health. The purpose of this study was to document the antioxidant properties of elderberry fruit extracts. The study included 9 different genotypes of elderberries (the species Sambucus javanica and S. nigra, and seven interspecific hybrids). The antioxidative activity of elderberry extracts was measured using DCFH-DA staining (2′, 7′-dichlorofluorescin diacetate) in vitro using a model macrophage cell line TLT. Fluorescence results were evaluated in different ways, since there was no specific way to calculate the intracellular antioxidant potential compared to the positive control or standard antioxidants. We found that fruits of the studied genotypes of elderberries had different intracellular antioxidant potential. The highest intracellular antioxidative potential was exhibited by Sambucus nigra (NI) and the lowest by the interspecific hybrid (JA × NI) × MIQ. The results can be very useful for further selection and breeding of elderberry hybrids. antioksidanti bezeg antioksidativna aktivnost DCFH-DA celice antioxidants elderberry DCFH-DA antioxidative potential cells true true false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2020-09-16 12:35:43 2020-09-29 09:35:56 2022-08-09 17:16:10 2020-09-30 03:23:04 2020 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:PZXFN6KD MAG_Arnus_David_2020.pdf MAG_Arnus_David_2020.pdf 1 393AFD62BC3147F1992D36243F2BFCFE 4781a54bbd8fb7aae2c580a92b18a54a8e5a7b10323066e44c2b5b0dca816ab7 e161a9aa-7c11-11eb-bb7a-00155d0001ca 20.500.12556/dkum/542e3e40-2938-4bff-af32-1aa319376881 https://dk.um.si/Dokument.php?lang=slv&id=146289 Fakulteta za kmetijstvo in biosistemske vede 0 0 0