20.500.12556/DKUM-78707 Osamitev in identifikacija psihrofilnih in psihrotolerantnih bakterij in gliv iz Triglavskega ledenika in ledenika pod Skuto Isolation and identification of psychrophilic and psychrotolerant bacteria and fungi from triglav and skuta glacier Ledeniki so dolgo veljali za abiotska okolja. Novejše raziskave pa kažejo, da so ledeniki posebni in dinamični mikrobni habitati, kjer uspevajo različni psihrofilni in psihotolerantni mikroorganizmi. Zaradi globalnega segrevanja se visokogorski ledeniki pospešeno talijo. S tem izginjajo okolja, v katerih so se preko tisočletij uspešno razvijale mikrobne združbe v relativno stabilnih pogojih. Mikroorganizmi, ki naseljujejo ledenike, so razvili vrsto prilagoditev na življenje pri nizkih temperaturah in na ciklična zamrzovanja in odtaljevanja. V Sloveniji trenutno obstajata dva ledenika. Triglavski ledenik leži v Julijskih Alpah, na severovzhodnem pobočju najvišjega vrha v državi, Triglava. Ledenik pod Skuto leži dobrih petdeset kilometrov vzhodneje ter nekaj 100 m južneje od Triglavskega ledenika. Vzorčenje na obeh ledenikih je potekalo v septembru 2018. Iz obeh ledenikov smo aseptično odvzeli tri vzorce ledu iz treh različnih lokacij, jih stalili in filtrirali ter filtre inkubirali na štirih različnih gojiščih primernih za rast gliv ter na enem gojišču za bakterije pri temperaturi 15 °C. Skupno smo osamili 181 glivnih izolatov, ki se uvrščajo v 19 rodov ter 66 bakterijskih izolatov, ki se uvrščajo v 22 rodov. Glaciers were long known as abiotic environments, but latest reseach shows that they are special and dynamic habitats where various psychrofilic and psychrotolerant organisms can thrive. Due to global warming highland glaciers are rapidly thawing, releasing enclosed cold-adapted microbial communities into the environment. Since glacial microbial communities were growing in relatively stable conditions, characterized by low temperature, few nutrients, surface exposure to UV-radiation, increased salinity in brine channels around ice crystals, many can't survive these changes. In Slovenia, only two glaciers (glacierets) remain. Triglav glacier is located in Julian Alps, on the northeast slope of the country's highest peak, Mt. Triglav. Skuta glacier, a bit further to the east is located in Kamnik-Savinja Alps. Sampling of ice was conducted on both glaciers in September 2018. Three samples on different locations on each glacier were taken and brought to the laboratory for further analyses. The samples were aseptically thawed and filtrated through membrane filters and incubated on different selective microbial culture media with temperature of 15 °C. We succsefully isolated and identificated 181 strains of fungi belonging to 19 genera and 66 bacterial strains belonging to 22 genera. Triglavski ledenik ledenik pod Skuto ledeniki psihrofili psihrotoleranti mikroorganizmi bakterije glive izolacija identifikacija PCR ITS. Triglav glacier Skuta glacier glaciers psychrophilic microorganisms psychrotolerant microorganisms bacteria fungi isolation identification PCR ITS. true true false [M. Jarčič] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2021-02-12 10:41:17 2021-03-23 10:31:17 2022-08-09 19:20:59 2021-02-24 03:26:26 2021 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.24/26:577.2(497.4)(043.2) 56658947 URN:SI:UM:DK:ZV9GGODM MAG_Jarcic_Masa_2021.pdf MAG_Jarcic_Masa_2021.pdf 1 A5E5044D37D42CF3484121E7C3E4E5E1 a2fd1287c2f8e0b226103f631de84a0641c1d96a85be3a7203fc57b5a3b4567b 21b6837e-7c12-11eb-bb7a-00155d0001ca 20.500.12556/dkum/7c596d9b-37aa-4181-bef8-8ff8a5aa39f4 https://dk.um.si/Dokument.php?lang=slv&id=149008 Fakulteta za naravoslovje in matematiko 0 0 0