20.500.12556/DKUM-78718 Esterifikacija maščobnih kislin z biokatalizatorjem vezanim na mezoporozni silikatni nosilec magistrsko delo Esterification of fatty acid using biocatalyst attached to mesoporous silicate support Reakcija spajanja maščobnih kislin in alkohola, pri kateri nastane produkt ester, ima pomembno vlogo v vsakdanjem življenju in prav tako v kemijski industriji. Produkt, alkilni ester, je najpogostejša vrsta biodizla. V tej raziskavi smo sintetizirali in karakterizirali biokatalizator, pri katerem smo kot nosilec uporabili mezoporozni silicijev dioksid (SBA-15) z veliko specifično površino in enakomerno velikostjo por. S postopkom sinteze smo vplivali na povečanje premera por SBA-15 z uporabo triizopropilbezena (TIPB). Kot ne-specifično povezovalno sredstvo za imobilizacijo encima na modificiran nosilec smo uporabili glutaraldehid. Učinkovitost imobilizacije lipaze na sintetizirani mezoporozni siliki smo preverili z reakcijo esterifikacije oleinske in miristinske kisline. Eksperimentalni del je potekal v avtomatiziranem reakcijskem sistemu. Preučevali smo vpliv ključnih procesnih parametrov temperature,  = (20, 25 in 30) °C , pH = (6, 7 in 8), množinskega razmerja kislina/alkohol in količine biokatalizatorja, na učinkovitost imobilizacije in esterifikacije. The combination of fatty acid and alchohol forms a product ester. Esters plays a significant role in everyday life and also in the chemical industry. The product alkyl ester is the most common type of biodiesel. In this study we synthesized and characterized a biocatalyst using mesoporous silica (SBA-15) as a support. The high surface area of mesoporous silica SBA-15 has been synthesized successfully by using triblock copolymer (P123) as a direct structure agent and tetraethyl orthosilicate (TEOS) as a precursor. 1,3,5-Triisopropylbenzene (TIPB) were used in order to increase ultralarge pore. Glutaraldehyde was used as a non-specific cross-linking agent for fixing the lipases on mesoporous silica. Enzyme was covalently bonded on the support. The efficiency of bonding was tested by well-known esterification of oleic and myristic acid. All experiments were carried out in the automated reaction system. We studied the influence of key process parameters: temperature, ϑ = (20, 25 and 30) ° C, pH = (6, 7 and 8), acid/alcohol ratio and amount of biocatalyst on immobilization and esterification efficiency. mezoporozni silikatni nosilec imobilizacija lipaza esterifikacija oleinska kislina miristinska kislina mesoporous silicate support immobilization lipase esterification oleic acid myristic acid true true false [K. Zečević] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2021-02-14 14:53:55 2021-03-31 16:13:35 2022-08-09 19:21:40 2021-02-15 03:22:21 2021 0 Maribor 0 Maribor IX, 43 f. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.095.13:546.284-31(043.2) 58691843 URN:SI:UM:DK:0EUDOSSG MAG_Zecevic_Katja_2021.pdf MAG_Zecevic_Katja_2021.pdf 1 DC04FC72612C916DD5F83F0BBCA4174D f4f7e3ef35ca70f36b1fd3792f5e33efcb55adb02ef3e8a8cc8c84cd2ae16d9d 81f04add-90bc-11eb-8842-00155d0001ca 20.500.12556/dkum/2e2cde86-2b33-4fda-8930-86fcb6cac964 https://dk.um.si/Dokument.php?lang=slv&id=149552 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0