20.500.12556/DKUM-79238 Vpliv dodanih citokininov (meta-topolin in BAP) na rast in razraščanje pri mikropropagaciji sliv (Prunus domestica L.) The Influence of Added Cytokinins (meta-Topolin and BAP) on Plum (Prunus domestica L.) Growth and Shoot Formation in Micropropagation V letu 2020 smo na Fakulteti za kmetijstvo in biosistemske vede Univerze v Mariboru pripravili poskus, v katerem smo ugotavljali vpliv dodanih citokininov (meta-topolin in BAP) in njihovih koncentracij na rast in razraščanje poganjkov ter na pojav vitrifi-kacije sliv v tkivni kulturi. Uporabili smo predhodno sterilizirane in vitro poganjke sliv. Pripravili smo 4 različna gojišča. Gojišče G0 je bilo kontrolno gojišče (brez doda-nih rastnih hormonov), gojišče G1 z dodatkom 0,01 mg/l NAA in 1,00 mg/l meta-topolina, gojišče G2 z dodatkom 0,01 mg/l NAA in 0,50 mg/l BAP in gojišče G3, ki smo mu dodali 0,01 mg/l NAA in 1,00 mg/l BAP. Ugotovili smo, da dodani citokinini pozi-tivno vplivajo na prirast mase sliv in število novih poganjkov. Največji povprečni pri-rast mase sliv je bil pri gojišču G2 (1,54 g), najmanjši pa pri kontrolnem gojišču G0 (0,13 g). Največje povprečno število poganjkov je bilo na gojišču G2 (5,37). Pri 5-odstotni stopnji tveganja ni bilo ugotovljenih statistično značilnih razlik glede vpliva koncentracije dodanih citokininov na prirast mase sliv in število poganjkov pri po-sameznem gojišču. Pojav vitrifikacije ni bil prisoten pri nobenem obravnavanju. In 2020, an experiment was prepared at the Faculty of Agriculture and Life Sciences of the University of Maribor, in which the effect of added cytokinins (meta-Topolin and BAP) and their concentrations on the growth, shoot formation and presence of vitrification on plums in tissue culture was determined. Presterilized in vitro plum shoots were used. Four different media were prepared. The G0 medium was a con-trol medium (without added growth hormones), the G1 medium with the addition of 0.01 mg/l NAA and 1.00 mg/l meta-Topolin (mT), the G2 medium with the addi-tion of 0.01 mg/l NAA and 0.50 mg/l BAP and the G3 medium to which we added 0.01 mg/l NAA and 1.00 mg/l BAP. The results indicate that the added cytokinins had a positive effect on tissue mass gain and on number of new shoots developed. The largest mean of plant tissue weight gain was in the G2 medium (1.54 g) and the smallest in the controlled medium G0 (0.13 g). The largest average number of shoots was in the G2 medium (5.37). At a 5% risk rate, no statistically significant differences had been found in the effect of the concentration of added cytokinins on plant tis-sue weight gain and number of shoots in each medium. The vitrification was not observed in any treatment. sliva mikropropagacija citokinini meta-topolin BAP plum micropropagation cytokinins meta-Topolin BAP true true false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2021-06-01 19:38:47 2021-06-17 13:22:40 2022-08-09 20:19:26 0000-00-00 00:00:00 2021 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 67372803 MAG_Salamun_Marko_2021.pdf MAG_Salamun_Marko_2021.pdf 1 8E848DCD5BFF7DC3E420A1DAD9A6EA49 b3233ebb1d2bc1fe0cf1853397a5911fff720a1375f99c54511de5a9a60e3c0f 081b9d42-c3eb-11eb-8f2c-00155d581298 20.500.12556/dkum/cc90f318-aed4-413b-85a6-d310bf53aefa https://dk.um.si/Dokument.php?lang=slv&id=150512 Fakulteta za kmetijstvo in biosistemske vede 0 0 0