20.500.12556/DKUM-79555 Hidroliza triacetina z uporabo encima lipaze diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje Hydrolysis of triacetin using lipase enzyme V diplomskem delu smo preučevali reakcijo hidrolize triacetina pri različnih pogojih. Reakcije smo najprej izvajali v fosfatnem pufru (c = 0,05 mol/L), pri pH = 7, kateremu smo dodali 1 mL 99 % triacetina. Reakcije so potekale pri (25, 30 in 35) °C in z različnimi količinami dodanega katalizatorja – encima lipaze (0,2; 0,4 in 0,6) g. Za primerjavo smo izvedli reakcije z 0,5 in 2 mL triacetina pri vseh treh temperaturah in 0,2 g lipaze. Na koncu smo se odločili izvesti dve reakciji pri najvišji temperaturi in največji količini dodane lipaze z 1 mL triacetina in fosfatnim pufrom s pH=6 in 8. Za spremljanje reakcij smo uporabili in- situ ReactIR 702L, podjetja Mettler Toledo. Ugotovili smo, da dosežemo pri reakciji izvedeni pri 35 °C, z 0,6 g dodane lipaze in pri pH=7 in 8 najvišjo presnovo. Iz rezultatov je razvidno, da je reakcijska hitrost višja pri višjem pH. Dobljene rezultate smo opisali z Michaelis-Menten-inim modelom. Povprečne Michaelis-Menten-ine konstante so 103,9; 104,8 in 238,3 mol/L in aktivacijske energije 104,2; 109,3 in 64,3 kJ/mol za reakcije z 0,2; 0,4 in 0,6 g dodane lipaze. Izračunane konstante proizvodnosti so manjše pri nižji temperaturi in manjši količini dodane lipaze. In the bachelor’s thesis, we studied the hydrolysis reaction of triacetin under different conditions. The reaction was carried out by the usage of phosphate buffer (c = 0,05 mol/L) at pH = 7, to which we added 1 mL of 99 % triacetin. Reactions took place at 25, 30 and 35 °C and with varying quantities of added catalyst – lipase enzyme (0,2; 0,4 and 0,6 g). For comparison, we carried out reactions with 0,5 and 2 mL of triacetin at all three temperatures and 0,2 g of lipase. In the end, we decided to perform two reactions at the highest temperature and the largest addition of lipase with 1 ml of triacetin and phosphate buffer at pH = 6 and pH = 8. For reaction observation, we used in situ ReactIR 702L from Mettler Toledo. We found out that the reactions at 35 °C with 0,6 g of lipase and pH = 7 and 8 had the most substantial conversion. The results also show that the reaction rates increase by the usage of pH = 8 buffer rather than the lower pH buffer. The obtained results were described with the Michaelis-Menten model. Average Michaelis-Menten constants were 103,9; 104,8 and 238,3 mol/L, and activation energies 104,2; 109,3 and 64,3 kJ/mol for 0,2; 0,4 and 0,6 g of added lipase. Obtained reaction rate constants were lower at lower temperatures and lower amounts of added lipase. triacetin hidroliza diacetin lipaza FTIR triacetin diacetin hydrolysis lipase FTIR true true false [N. Belina] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2021-07-20 13:41:05 2021-09-08 15:53:13 2022-08-09 21:43:18 0000-00-00 00:00:00 2021 0 Maribor 0 Maribor X, 44 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.094.941(043.2) 75916803 VS_Belina_Nina_2021.pdf VS_Belina_Nina_2021.pdf 1 104E360E87BC4A3F3BCA37B2386AB707 013fd0bd3a87fa86a312572dec4ed4a8285721b5e52855ffb5822ee740fc05a5 48d001f5-0eda-11ec-8ed2-00155d581298 20.500.12556/dkum/3a94351a-e9a5-48b6-8017-076311274243 https://dk.um.si/Dokument.php?lang=slv&id=153134 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0