20.500.12556/DKUM-80201 Določitev optimalnih pogojev superkritične ekstrakcije s programom Design Expert in bioaktivnih komponent arnike (Arnica montana) magistrsko delo Determination of optimal conditions of supercritical extraction with program design expert and bioactive components of arnica (arnica montana) Rastlinske bioaktivne spojine (antioksidanti, totalni fenoli, proantocianidini, flavonoidi, itd.) so sekundarni metaboliti, ki jih proizvajajo rastline in izzovejo farmakološke ali toksične učinke pri človeku in živalih. Postopek priprave in izbira topila vplivata na kvaliteto in kvantiteto ekstrakta oz. komponent v ekstraktu. V magistrski nalogi smo raziskali rastlino Arnico Montano, ki ima v farmacevtski in kozmetični industriji velik potencial. Osredotočili smo se na postopek superkritične ekstrakcije in določili optimalne pogoje superkritične ekstrakcije arnike s CO2 na podagi izrisanega eksperimentalnega načrta, programa Design Expert Pro 12. Ugotovili smo, da višja kot sta temperatura in tlak, višji je izkoristek ekstrakcije. Pridobljenim ekstraktom smo s spektrofotometrično metodo determinirali biološko aktivne komponente (antioksidanti, totalni fenoli, proantocianidini, totalni flavonoidi), katerih prisotnost naj bi vplivala na protivnetni, antiradikalni, antibakterijski, protiglivični in antioksidativni potencial. Rezultati so nam pokazali, da ekstrakti, ki so bili pridobljeni pri višji temperaturi ter tlaku, vsebujejo višje vrednosti biološko aktivnih komponent. Slednjim smo nato določali še protimikrobni potencial, ki smo ga potrdili le ekstraktu 8 z najvišjo vsebnostjo antioksidantov ter totalnih flavonoidov, pridobljenem pri tlaku 100 bar in temperaturi 60 C. Plant bioactive compounds (antioxidants, total phenols, proanthocyanidins, flavonoids, etc.) are secondary metabolites produced by plants and cause pharmacological or toxic effects in humans and animals. The preparation process and the choice of solvent affect the quality and quantity of the extract or. components in the extract. In our master's thesis, we researched the Arnico Montano plant, which has great potential in the pharmaceutical and cosmetic industries. We focused on the supercritical extraction process and determined the optimal conditions for supercritical extraction of arnica with CO2 on the basis of a plotted experimental plan, Design Expert Pro 12. We found that the higher the temperature and pressure, the higher the extraction efficiency. The obtained extracts were determined by the spectrophotometric method of biologically active components (antioxidants, total phenols, proanthocyanidins, total flavonoids), the presence of which is expected to affect the anti-inflammatory, antiradical, antibacterial, antifungal and antioxidant potential. The results showed us that the extracts obtained at higher temperature and pressure contain higher values of biologically active components. The latter were then determined for their antimicrobial potential, which was confirmed only in extract 8 with the highest content of antioxidants and total flavonoids, obtained at a pressure of 100 bar and a temperature of 60 C. gorska arnika antioksidativnost fenolne spojine proantocianidini protimikrobni potencial mountain arnica antioxidant activity phenolic compounds proanthocyanidins antimicrobial potential true true false [P. Tržan] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2021-09-01 16:47:40 2021-09-22 06:33:55 2022-08-09 22:37:21 0000-00-00 00:00:00 2021 0 Maribor 0 Maribor XI, 40 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 604.4:615.33(043.2) 77464323 MAG_Trzan_Primoz_2021.pdf MAG_Trzan_Primoz_2021.pdf 1 E9A9C9CBB6A2CE54F2268B56667CB041 4c97dbd56f4602954093c3984d83e58a659043844f5da69b73a2f00f6e673f78 fd074d87-1577-11ec-8600-00155d581298 20.500.12556/dkum/b1254e18-67e6-48ce-a80f-dd085bcb6ea6 https://dk.um.si/Dokument.php?lang=slv&id=153848 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0