20.500.12556/DKUM-80315 Funkcionalizacija celuloznih vlaken z uporabo protimikrobnih sredstev in/ali probiotikov za novo generacijo tamponov magistrsko delo Functionalization of celulose fibers using antimicrobial agents and/or probiotics for new generation of tampons To magistrsko delo temelji na razvoju in funkcionalizaciji tamponov z dodano vrednostjo. Tampone smo funkcionalizirali s probiotično bakterijo Lactobacillus. gasseri K7. S pomočjo tamponov nove generacije želimo tako pomagati ženskam pri preprečevanju okužb z bakterijami in glivami na način ohranjanjanje zdrave vaginalne mikrobiote v času menstrualnih krvavitev. Lastnosti funkcionaliziranih tamponov bodo ovrednotene s pomočjo različnih testnih metod, ki temeljijo na uporabnih lastnostih tamponov. Magistrska naloga zajema nanos liofiliziranih in svežih probiotikov, ujetih v hidroksi β ciklodekstrine s pomočjo tehnike elektropredenja na nosilni material. V tem magistrskem delu sem probiotike, tako sveže kot tudi liofilizirane ujela v ciklodekstrine in iz dobljene koloidne raztopine izpredla kot nanovlakna z elektropredenjem, najprej na polipropilen, na koncu pa še na tamponski trak podjetja Tosama d.o.o. Funkcionaliziran material sem analizirala s pomočjo metod (elementna sestava: XPS, ATR-FTIR, morfologija: SEM mikroskopija, bioaktivnost: določanje antioksidativnosti po metodi ABTS in sproščanje probiotikov ). Rezultati uporabljenih analiz kažejo, da se pri elektropredenju tvorijo nanovlakna, ki se naložijo na površino nosilnega materiala. Dokazali smo tudi, da se probiotiki ujamejo v ciklodekstrine in se obdržijo tako na polipropilenski kopreni, kot tudi na bombažnem tamponskem traku. Sproščanje probiotikov iz obeh uporabljenih nosilnih materialov je pokazalo, da se bakterija sprošča iz materiala še do 4 ure, kar je zelo obetajoč rezultat Antioksidativni potencial smo merili z namenom preverjanja protivnetnega učinka funkcionaliziranega nosilnega materiala. Rezultati so pokazali da imajo le - tega vzorci, katere smo funkcionalizirali z liofiliziranimi bakterijami. This graduation thesis is based on the development and functionalisation of tampons with added value. The tampons will be functionalised with the probiotic bacteria Lb. gasseri K7. The next generation tampons are intended to help women prevent bacterial and fungal infections by sustaining healthy vaginal flora during menstrual bleeding. The properties of functionalised tampons will be evaluated using various test methods based on the functional properties of tampons. The graduation thesis covers the application of lyophilized and fresh probiotics enveloped in hydroxy β cyclodextrins using the technique of electrospinning on the carrier material. During this graduation thesis, probiotics, both fresh and lyophilized, were enveloped in cyclodextrins; the obtained colloidal solution was spun as nanofibers by electrospinning, first on polypropylene, and finally on the tampon tape by the company Tosama d.o.o. The functionalised material was analysed using the following methods (elemental composition: XPS, ATR-FTIR, morphology: SEM microscopy, bioactivity: determination of antioxidant properties using the ABTS method and the release of probiotics). The results of the methods used show that nano fibres are being produced during electrospinning; these are deposited on the surface of the carrier material. The thesis also proves that the probiotics get trapped in cyclodextrins and are preserved on the polypropylene as well as the cotton tampon tape. The release of probiotics from both carrier materials used has shown that the bacterium was being released from the material for up to 4 hours, which is a very promising result. The antioxidant potential was measured in order to check the anti-inflammatory effect of the functionalised carrier material. The results show this effect present in the samples which had been functionalised by lyophilized bacteria. funkcionalizacja tamponi probiotiki elektropredenje ciklodekstrini functionalization tampons probiotics electrospinning cyclodextrins true true false [L. Spasković] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2021-09-07 09:27:12 2021-10-04 11:25:35 2022-08-09 22:46:31 0000-00-00 00:00:00 2021 0 Maribor 0 Maribor IX, 61 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 [604.4:615.33]:677.467(043.2) 99768835 MAG_Spaskovic_Larisa_2021.pdf MAG_Spaskovic_Larisa_2021.pdf 1 BBABF4F2F0C58361352B9BFD38679B65 45cb3071d9b81010091f38331f82db8af547cc8c8420842c39b3c2695f284db6 6041bffc-1f65-11ec-8fe5-00155d000105 20.500.12556/dkum/10b30366-ae9b-4fa0-8d36-791621bc6bcc https://dk.um.si/Dokument.php?lang=slv&id=154484 Fakulteta za strojništvo 0 0 0