20.500.12556/DKUM-80378 Kontrolirano sproščanje resveratrola v simulacijskih tekočinah magistrsko delo Controlled release of resveratrol in simulation fluids Vse pogosteje se v prehranskih dopolnilih uporabljajo aktivne komponente, pripravljene iz naravnih materialov. Kakšna je njihova biodostopnost v določenem organizmu, se preverja s testi raztapljanja, ki predstavljajo pomemben preizkus za kontrolirano sproščanje učinkovine in vivo ter za kontrolo kvalitete, kot tudi kontrolo v določenih fazah razvoja določene farmacevtske oblike. Pri našem delu smo proučevali sproščanje resveratrola iz filmsko obložene tablete, kapsule, standarda in lastne formulacije v simulacijskih tekočinah. Za simulacijske tekočine smo izbrali 0,1 M HCl in destilirano vodo. Zaradi šibko kislih lastnosti učinkovine smo predvidevali, da bo njeno raztapljanje hitrejše pri višjem pH-ju, pri nižjem pH-ju pa se bo učinkovina sproščala počasneje. Kljub temu smo za simulacijsko tekočino izbrali 0,1 M HCl, da bi ponazorili pogoje v želodcu. Raztapljanje resveratrola v 0,1 M HCl ni bilo uspešno, saj je bila pri vseh formulacijah z izjemo kapsule njegova topnost nezaznavna. Raztapljanje resveratrola v destilirani vodi je bilo nad pričakovanim, saj glede na podatke iz literature ni topen v vodi. Neraziskano ostaja predvsem področje stranskih produktov, ki nastanejo pri raztapljanju resveratrola pod določenimi pogoji v organizmu (pH, topnost, stabilnost). Želodčna kislina mora imeti pH med 1 in 3, kar je zelo kislo. Resveratrol kljub temu, da naj bi bil stabilen do pH-ja 6, v našem primeru ni bil stabilen v 0,1M HCl. V prihodnje je treba raziskati, kateri nosilec bi bil dober, da bi bil resveratrol stabilnejši v pogojih, kakršni so prisotni v organizmu. Increasingly, active components made from natural materials are used in dietary supplements. What is their bioavailability in a particular organism, which is verified by dissolution tests, which is an important test for the controlled release of the active substance in vivo and for quality control, as well as control at certain stages of development of a particular pharmaceutical form. In our experiments, we studied the release of resveratrol from different forms (a tablet, capsule, standard and resveratrol's own formulation) in simulation liquids. 0,1 M HCl and distilled water were selected as the simulation liquids. Due to the weakly acidic properties of the resveratrol, we assumed that its dissolution would be faster at a higher pH and slower at a lower pH. Despite its low pH, 0,1 M HCl was still chosen as the simulation liquid in order to illustrate conditions which are present in the human stomach. Dissolution of resveratrol in 0,1 M HCl was not successful – its solubility was undetectable in all formulations, with a capsule as the only exception. On the other hand, dissolution of resveratrol in distilled water was better than expected, since according to the data from the literature, resveratrol is insoluble in water. The dissolution of resveratrol's byproducts, which are formed in the body under certain conditions (pH, solubility, stability), is still not investigated enough. Stomach acid has a pH between 1 and 3, which is very acidic. Despite the fact that resveratrol should be stable at pH 6, it was not stable in 0,1 M HCl in our experiments. In the future, it would be especially beneficiary to investigate which carrier would be the most appropriate to use to improve resveratrol's stability under certain conditions, which are present inside human bodies. resveratrol raztapljanje stresni testi PGSS LC-MS/MS resveratrol dissolution stress test PGSS LC-MS/MS true true false [P. Bandur] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2021-09-10 00:14:27 2021-10-06 08:29:38 2022-08-09 22:51:16 0000-00-00 00:00:00 2021 0 Maribor 0 Maribor IX, 46 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 544.351:615.015.15(043.2) 80886787 MAG_Bandur_Patricija_2021.pdf MAG_Bandur_Patricija_2021.pdf 1 1B7C3A2A6799695381818C492ACD5CB5 01565344c9312acd9ebfe11929d3419896e07cae743d92b5385f6f0285b533a4 c14488ce-21d5-11ec-b66a-00155d000105 20.500.12556/dkum/49ca10f7-12da-43e2-b84f-5aaa944cfad0 https://dk.um.si/Dokument.php?lang=slv&id=154716 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0