20.500.12556/DKUM-81626 Okolju prijazno pridobivanje ekstraktov iz rožmarina (rosmarinus officinalis l.) z globoko evtektičnimi topili in ocena njihove antioksidativne učinkovitosti magistrsko delo Environmentally friendly preparation of rosemary (rosmarinus officinalis l.) extracts with deep eutectic solvents and evaluation of their antioxidant activity Globoko evtektična topila predstavljajo zeleno alternativo konvencionalnim organskim topilom za ekstrakcijo bioaktivnih učinkovin. V sklopu magistrskega dela smo za namen ekstrakcije antioksidativnih spojin iz rožmarina sintetizirali šest različnih globoko evtektičnih topil, pri čemer so vsi kot akceptorja vodikove vezi vsebovali holin klorid, kot donorji vodikove vezi pa so se izmenjevali etilen glikol, 1,2-propandiol, ocetna kislina, mlečna kislina, urea in fruktoza, kot primerjavo s konvencionalim topilom pa smo uporabili raztopini različnih koncentracij etanola in metanola. Za pripravo vzorcev smo uporabili tehniko matrično trdno-fazne disperzije, za identifikacijo in kvantifikacijo izbranih spojin pa smo uporabili metodo tekočinske kromatografije ob uporabi UV detektorja (HPLC-UV). Dobljenim ekstraktom smo določili še antioksidativno delovanje s TPC, FRAP in DPPH testi. Vsi antioksidativni testi so pokazali, da je imel vzorec, pri katerem smo uporabili mlečno kislino kot HBD, najvišjo antioksidativno aktivnost, temu pa sta sledila vzorca, pridobljena s 70 % etanolom in z DES topilom na osnovi ocetne kisline. Dobljene rezultate smo primerjali s podobno raziskavo na področju ekstrakcij fenolnih spojin iz rožmarina z uporabo globoko evtektičnih topil, pri čemer smo njihove rezultate presegli. S tem smo razvili in vpeljali novo, hitro, bolj učinkovito in cenovno ugodno ekstrakcijsko metodo ob uporabi globoko evtektičnih topil za pripravo rastlinskih ekstraktov z visoko vsebnostjo aktivnih učinkovin iz rožmarina. Deep eutectic solvents have been explored as green alternative to conventional organic solvents to extract natural bioactive compounds. In this thesis, six deep eutectic solvents were synthesized for the purpose of extracting antioxidant compounds from rosemary, all of which contained choline chloride as hydrogen bond acceptor, and ethylene glycol, 1,2-propanediol, acetic acid, lactic acid, urea and fructose as hydrogen bond donor. As a comparison with a conventional solvent, solutions of different ethanol and methanol concentrations were used. Matrix solid phase dispersion method of sample preparation was used and the high-performance liquid chromatography method with a UV detector (HPLC-UV) was used to identify and quantify the selected compounds. Finally, the extracts obtained were evaluated for their antioxidant activity by TPC, FRAP and DPPH assays. All antioxidant tests showed that the sample in which lactic acid was used as HBD had the highest antioxidant activity, followed by samples obtained with 70% ethanol and with DES solvent, based on acetic acid. The obtained results were compared with a similar study in the field of extraction of phenolic compounds from rosemary using deep eutectic solvents and exceeded their results. Thus, we have developed and introduced a new, fast, more efficient and cost-effective extraction method using deep eutectic solvents fort he preparation of plant extracts with high content of active compounds from rosemary. Globoko evtektična topila rožmarin HPLC TPC FRAP DPPH Deep eutectic solvents rosemary HPLC TPC FRAP DPPH true true false [Ž. Koštomaj] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2022-04-29 13:11:21 2022-05-25 07:39:06 2022-08-10 00:40:22 0000-00-00 00:00:00 2022 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (IX,54 f.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 544.032.732:[66.061.3:582.943.16](043.2) 111192579 MAG_Kostomaj_Zan_2022.pdf MAG_Kostomaj_Zan_2022.pdf 1 6D8E55FEDECE672872686D40DF7EB6D0 6e5b8c58e6768bbe93e80d22761ab1957b2b329ad1a072c309e62b0cb945c8dc 0236c289-db87-11ec-bc9e-00155d000105 20.500.12556/dkum/df127411-7223-4ab8-a0d8-bfddf3f9dca4 https://dk.um.si/Dokument.php?lang=slv&id=158036 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0