20.500.12556/DKUM-81819 Izolacija učinkovin industrijske konoplje ter sinergistični učinek mešanic materialov družine Zingiberaceae magistrsko delo Isolation of biologically active components from hemp and analysis of the synergistic effect of mixtures of the family zingiberaceae Namen magistrskega dela je raziskati vpliv mešanic industrijske konoplje z ingverjem, kurkumo in kardamomom. Ekstrakti so bili pridobljeni iz posameznih naravnih materialov in kot mešanice industrijske konoplje s posameznimi člani družine Zingiberaceae v razmerju 1 : 1. Pridobivali smo jih z dvema metodama, s superkritično ekstrakcijo pri p = 250 bar in T = 50 °C ter z ultrazvočno ekstrakcijo ob uporabi etanola kot topila. Višje izkoristke ekstrakcij posameznih rastlinskih materialov smo dosegli v primeru ultrazvočne ekstrakcije, z izjemo industrijske konoplje, ki se je v večji meri izločila pri superkritični ekstrakciji. Prav tako so pri omenjeni vrsti ekstrakcije mešanice industrijske konoplje z ingverjem, kurkumo in kardamomom dosegale višje izkoristke. Hkrati smo pri superkritičnem pridobivanju ekstraktov spremljali kinetiko, kjer so se najhitreje ekstrahirale komponente kurkume, medtem ko so komponente industrijske konoplje za izločitev potrebovale največ časa. Sledila je statistična obdelava podatkov, kjer smo s programom ANOVA potrdili, da ne prihaja do statistično pomembnih razlik pri antioksidantih in totalnih fenolih, hkrati pa opazili, da je prišlo do sinergističnega učinka antioksidantov v primeru mešanice industrijske konoplje in kardamoma. Pri omenjeni mešanici se prav tako izloči več alfa terpinil acetata kot pri samem kardamomu. Pozitiven vpliv mešanic smo zaznali še v primeru industrijske konoplje in ingverja, kjer se je izločilo več komponente CBD. Nadalje nam je s Pearsonovim korelacijskim testom uspeli dokazati močno statistično značilno korelacijo med antioksidanti in totalnimi fenoli, antioksidanti in biološko aktivnimi komponentami ingverja ter med samima komponentama ingverja (6-gingerolom in 6-shogaolom). The goal of the Master’s thesis is to research the effect of blends of industrial hemp with ginger, turmeric and cardamom. The extracts were obtained from different natural materials and from blends of industrial hemp with single members of the Zingiberaceae family in a 1:1 ratio. They were obtained with two methods, i.e. supercritical extraction at p = 250 bar and T = 50 °C, and ultrasonic extraction using ethanol as a solvent. A higher rate of extraction of the single plant materials was obtained with ultrasonic extraction, with the exception of industrial hemp, which was released at a higher rate with supercritical extraction. With the supercritical extraction method the rate of extraction was higher also for industrial hemp blends with ginger, turmeric and cardamom. At the same time, we monitored the kinetics of supercritical extraction, where the extraction of turmeric compounds occurred the fastest, whereas the extraction of industrial hemp compounds took the longest. The extractions were followed by the processing of data, which was carried out with the ANOVA software and confirmed that there are no statistically significant differences in antioxidants and total phenols. At the same time, we observed a synergistic effect in the blend of industrial hemp and cardamom. Furthermore, in this blend more alpha-terpinyl acetate is released than in pure cardamom. A positive effect of blends was observed also in the industrial hemp-ginger blend, where more of the CBD component was released. Moreover, with the Pearson correlation test we proved a strong statistically significant correlation between antioxidants and total phenols, between antioxidants and biologically active compounds of ginger, as well as between the single compounds of ginger (6-gingerol and 6-shogaol). Industrijska konoplja Zingiberaceae mešanice kinetika ekstrakcij sinergistični učinek industrial hemp Zingiberaceae blends extraction kinetics synergistic effect. true true false [S. Sedonja] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2022-06-03 11:21:31 2022-07-13 14:12:47 2022-08-10 00:53:48 0000-00-00 00:00:00 2022 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (XI, 49 f.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.061.3:633.522(043.2) 116236803 MAG_Sedonja_Simona_2022.pdf MAG_Sedonja_Simona_2022.pdf 1 13B006F9E509964CA76D02D783A473D2 cfaa67d827020cd85f4983a2a49f05b7a439cb8b5c1f9e2c3067f6f672e325ea dd88f76b-028f-11ed-809b-00155d000105 20.500.12556/dkum/722a4411-78c3-4ded-b66c-aa79dd6e33c5 https://dk.um.si/Dokument.php?lang=slv&id=159360 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0