20.500.12556/DKUM-81933 Protein-repellent surface coatings made of polycaprolactone-polysaccharide blends master's thesis Protein odbojni površinski sloji narejeni iz polikaprolakton-polisaharid zmesi This work is focused on surface characterization and fibrinogen adsorption studies of polycaprolactone/chondroitin sulfate (tetrabutylammonium hydroxide) blends. Mixed polymer solutions were spin-coated to form two-phase thin films, which exhibit various patterns depending on the mass ratio of the spin-coating solution. Stability in the aqueous media, however, presents an uncertainty, due to the solubility of chondroitin sulfate (tetrabutylammonium hydroxide) in water. In spite of this, it is observed that the introduction of chondroitin sulfate (tetrabutylammonium hydroxide) in the film increases the repulsion of fibrinogen. Two methods frequently used for polymer characterization, mass determination using Quartz Crystal Microbalance with Dissipation monitoring and the zeta potential measurement, were found to be comparable techniques when studying protein adsorption and complementary techniques when analysing the stability of thin films. Magistrsko delo raziskuje površinske lastnosti polikaprolakton-polisaharid zmesi in njihov vpliv na adsorpcijo fibrinogena. Dvofazni tanki filmi so bili uspešno pripravljeni iz mešane polimerne raztopine z uporabo »spin coating« metode in tvorijo različne vzorce, odvisno od masnega razmerja polimerov. Stabilnost filmov v vodnem mediju predstavlja negotovost zaradi topnosti hondroitin sulfata (tetrabutilamonijevega hidroksida) v vodi. Kljub temu se v povprečju ob vnosu hondroitin sulfata (tetrabutilamonijevega hidroksida) v film kaže povečan odboj fibrinogena. Dve metodi, ki se pogosto uporabljata za karakterizacijo polimerov, merjenje mase s kremenovo mikrotehtnico z disipacijo in merjenje zeta potenciala, sta se izkazali kot primerljivi tehniki za preučevanje adsorpcije beljakovin in komplementarni tehniki za analizo stabilnosti tankih filmov. polycaprolactone chondroitin sulfate zeta potential QCM-D fibrinogen adsorption polikaprolakton hondroitin sulfat zeta potencial QCM-D fibrinogen adsorpcija true true false [J. Marko] Angleški jezik Slovenski jezik Magistrsko delo/naloga 2022-06-20 11:06:56 2022-07-15 14:24:20 2022-08-10 01:00:20 0000-00-00 00:00:00 2022 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (IX, 42 f.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 544.725:547.96(043.2) 116239363 MAG_Marko_Jan_2022.pdf MAG_Marko_Jan_2022.pdf 1 DC8D435F93D94F7028B9DBFACF6DFF20 8a5638d96af2b5786f0e83d03e7409ae15199fc47b5c8a95516891752dfc0856 661a5ecb-0423-11ed-8d7f-00155d000105 20.500.12556/dkum/bd4e76ea-5f13-4bcf-845f-571e6116f686 https://dk.um.si/Dokument.php?lang=slv&id=159418 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0