20.500.12556/DKUM-82194 Določitev terapevtske koncentracije ekstrakta trpotca v 3D tiskanem nosilnem sistemu za optimalni učinek na rast kožnih celic magistrsko delo Determination of therapeutic concentration of plantain extract in a 3D printed scaffolds for optimal effect on skin cell growth V magistrskem delu smo podrobneje raziskali vpliv ekstraktov širokolistnega (Plantago major L.) in ozkolistnega (Plantago lanceolata L.) trpotca na rast kožnih celic. Ekstrakte obeh vrst trpotca smo ekstrahirali z različnimi topili in jih določili. V ekstraktih smo s tekočinsko kromatografijo visoke ločljivosti potrdili prisotnost avkubina, katalpola, verbaskozida in plantamajozida. Vsebnost celokupnih kemijskih spojin v ekstraktih smo določili s spektroskopskimi metodami ter njihovo antioksidativno delovanje z DPPH-metodo. Kinetiko sproščanja ekstraktov iz 3D-tiskanih obližev smo določili na podlagi in vitro testiranja sproščanja verbaskozida iz 3D-tiskanih obližev na avtomatiziranem sistemu Franzovih difuzijskih celic. Na podlagi dobljenih rezultatov ter rezultatov MTT-testa, pri katerem smo določili vpliv ekstraktov na viabilnost celic, smo v 3D-tiskane obliže vgradili metanolni ekstrakt širokolistnega trpotca ter etanolni ekstrakt ozkolistnega trpotca. V primeru obeh vrst trpotca smo določili optimalni masni delež, ki je znašal 0,50 % ekstrakta na maso 3D-tiskanega obliža. Relativna viabilnost je bila v primeru 3D-tiskanega obliža s širokolistnim trpotcem 1,39 in 1,49 v primeru ekstrakta ozkolistnega trpotca. Z MTT-testom smo tako dokazali biokompatibilnost vgrajenih ekstraktov ter njihov spodbujevalni vpliv na viabilnost in proliferacijo fibroblastov. Z analizo površine 3D-tiskanih obližev z ali brez vgrajenega ekstrakta trpotca, ki smo jo naredili z vrstičnim elektronskim mikroskopom, smo potrdili vpliv ekstraktov na spremembo morfologije obližev. S testom degradacije in testom navzemanja vlage smo pokazali, da so obliži biorazgradljivi in da imajo ustrezen profil nabrekanja ter tako potrdili njihov potencialni terapevtski učinek in uporabo za celjenje ran. In this work, the effect of extracts from broad-leaved plantain (Plantago major L.) and narrow-leaved plantain (Plantago lanceolata L.) on skin cell growth was studied in detail. Extracts from both Plantago species were extracted with different solvents and were determined. The presence of aucubin, catalpol, verbascoside, and plantamajoside in the extracts was confirmed by high-performance liquid chromatography. The content of total chemical compounds in the extracts was determined by spectroscopic methods and their antioxidant activity by the DPPH method. The release kinetics of the extracts from the 3D-printed patches was determined by in vitro release assays of verbascoside from the 3D-printed patches using an automated Franz diffusion cell system. Based on the results obtained and the results of the MTT assay, where we were interested in the effect of the extracts on cell viability, we incorporated a methanolic extract of broad-leaved plantain and an ethanolic extract of narrow-leaved plantain into the 3D-printed patches. For both Plantago species, we determined the optimal extract mass fraction, which was 0.50% by weight of extract per weight of the 3D-printed patch. The relative viability was 1.39 in the case of the 3D-printed broad-leaved plantain patch and 1.49 in the case of the narrow-leaved plantain extract. Thus, the biocompatibility of the incorporated extracts and their stimulatory effect on viability and fibroblast proliferation were demonstrated by the MTT assay. By analysing the surface of the 3D-printed patches with or without the incorporated plantain extracts using a scanning electron microscope, we confirmed the effect of the extracts on changing the morphology of the patches. The degradation and moisture absorption test showed that the patches were biodegradable and had an adequate swelling profile, confirming their potential therapeutic effect and use for wound healing. širokolistni trpotec ozkolistni trpotec aktivne učinkovine 3D-tiskan nosilni sistem terapevtska koncentracija rast kožnih celic broad-leaved plantain narrow-leaved plantain active ingredients 3D-printed scaffolds therapeutic concentration skin cell growth true true false [K. L. Konda] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2022-07-28 00:46:40 2022-11-03 15:16:09 2022-12-13 03:07:16 0000-00-00 00:00:00 2022 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (XII, 63 f.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 [66.061:582.944.2]:616-001.4(043.2) 133439747 MAG_Konda_Klara_Laura_2022.pdf MAG_Konda_Klara_Laura_2022.pdf 1 FB30AA374B1DD328F4C0817EF8289824 2bd7930747420bdf64aef75d3228650f955e201cb208d0c40871c30eb12a3a95 3bb1bc4c-5af7-11ed-bc73-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=164240 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0