20.500.12556/DKUM-82625 Salivary gland adaptation to dietary inclusion of hydrolysable tannins in boars The ingestion of hydrolysable tannins as a potential nutrient to reduce boar odor in entire males results in the significant enlargement of parotid glands (parotidomegaly). The objective of this study was to characterize the effects of different levels of hydrolysable tannins in the diet of fattening boars (n = 24) on salivary gland morphology and proline-rich protein (PRP) expression at the histological level. Four treatment groups of pigs (n = 6 per group) were fed either a control (T0) or experimental diet, where the T0 diet was supplemented with 1% (T1), 2% (T2), or 3% (T3) of the hydrolysable tannin-rich extract Farmatan®. After slaughter, the parotid and mandibular glands of the experimental pigs were harvested and dissected for staining using Goldner’s Trichrome method, and immunohistochemical studies with antibodies against PRPs. Morphometric analysis was performed on microtome sections of both salivary glands, to measure the acinar area, the lobular area, the area of the secretory ductal cells, and the sizes of glandular cells and their nuclei. Histological assessment revealed that significant parotidomegaly was only present in the T3 group, based on the presence of larger glandular lobules, acinar areas, and their higher nucleus to cytoplasm ratio. The immunohistochemical method, supported by color intensity measurements, indicated significant increases in basic PRPs (PRB2) in the T3 and acidic PRPs (PRH1/2) in the T1 groups. Tannin upplementation did not affect the histo-morphological properties of the mandibular gland. This study confirms that pigs can adapt to a tannin-rich diet by making structural changes in their parotid salivary gland, indicating its higher functional activity. pigs dietary supplements tannins parotid gland mandibular gland immunohistochemistry histology proline-rich proteins (PRP) swine true false true MDPI AG Angleški jezik Slovenski jezik https://www.mdpi.com/journal/animals © 2022 by the authors Članek v reviji 2022-08-27 08:36:53 2023-07-07 14:49:42 2025-03-27 08:49:56 0000-00-00 00:00:00 2022 0 0 art. 2171, str. 1-15 št.. 17 Vol. 12 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-06-21 2022-08-19 2022-08-24 636.09:591 119067139 10.3390/ani12172171 2076-2615 RAZ_Mavri_Masa_2022.pdf RAZ_Mavri_Masa_2022.pdf 1 A1F00E577021D470E79F8031BA6E35AF cf981612909deec0399a11fafd1891c574bf28b50aeb2b78a3faae6189bee72d 52a40331-1ef9-11ee-b08f-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=168635 https://www.mdpi.com/2076-2615/12/17/2171 0 a5eb8fcf-25d2-11ed-ae97-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=160941 Fakulteta za elektrotehniko, računalništvo in informatiko 0 0 0