20.500.12556/DKUM-83895 Določanje fizikalno kemijskih lastnosti dostavnih sistemov s kontroliranim sproščanjem naravnih učinkovin magistrsko delo Determination of phisicochemicals properties of delivery systems with controlled release of natural active components Priljubljenost naravnih učinkovin iz rastlin je v velikem porastu, saj ima veliko aktivnih spojin številne antioksidativne, protivnetne, protibakterijske, protirakave učinke, kar predstavlja dragocen vir v medicini. Za naše raziskovanje smo si izbrali naravna materiala zeleno kavo in origano, katera vsebujeta aktivne učinkovine, ki imajo številne pozitivne vplive na zdravje ljudi. Pri kavi lahko izpostavimo klorogensko kislino ter kofein, pri origanu pa timol in karvakrol. Pripravili smo si organogele, za katere smo uporabili 3 različna olja (kokosovo, olivno in konopljino) ter 2 različna organogelatorja (stearinsko kislino in etil celulozo). Ugotovili smo, da v primeru uporabe konopljinega in olivnega olja ter gelatorjev stearinske kisline (SA) in etil celuloze (EC) pri pripravi organogelov ni bilo nobenih težav, medtem ko s kokosovim oljem in gelatorjem EC ustreznega organogela nismo dobili. Pri formulaciji ekstrakta kave v organogele smo ugotovili, da so samo formulacije z EC neenakomerno zgelirale in sicer so nastali skupki gelov. Pri formulaciji ekstrakta origana v organogele pa so naše formulacije bile neustrezne, izjema je bil organogel, pripravljen iz konopljinega olja in EC, ki je vseboval 10 % ekstrakta. Izvedli smo tudi formulacijo čistih komponent v organogele. V primeru timola, so nam formulacije organogelov pripravljene iz olivnega olja uspešno zgelirale. Tudi s kokosovim in konopljinim oljem smo dobili uspešno zgelirane organogele, z izjemo ene formulacije pri vsakem olju. Pri formuliranju karvakrola so nam formulacije organogelov pripravljene iz konopljinega olja uspešno zgelirale, medtem ko pri uporabi kokosovega in olivnega olja geliranje v enem vzorcu obeh formulacij ni bilo zadovoljivo. Nazadnje smo formulirali v organogele čisto komponento klorogensko kislino, kjer smo za vse formulacije dobili ustrezno gelacijo. Pri preverjanju reoloških lastnosti, smo ugotovili, da so se krivulje viskoznosti skoraj ujemale z obnašanjem krivulj pretoka za naše organogele in sicer ob splošnem zmanjševanje viskoznosti z naraščajočo strižno hitrostjo. Za zaključek smo izvedli raztapljanje različnih formulacij pripravljenih organogelov v 0,1 M HCl. Vzorce smo analizirali s pomočjo LC-MS/MS in GC-MS. Ugotovili smo, da je pri sproščanju različnih učinkovin najboljše rezultate dosegla klorogenska kislina, primerne krivulje vsebnosti za kofein in klorogensko kislino pa smo dobili tudi z raztapljanjem formulacij organogelov z ekstraktom kave. Uporaba drugih formulacij ni pokazala ustreznih rezultatov. The popularity of natural active components from plants is on the rise, as many active compounds have numerous antioxidant, anti-inflammatory, antibacterial, anti-cancer effects, which represents a valuable source in medicine. For our research, we chose the natural active ingredients green coffee and oregano, which contain active components with many positive effects on human health. Chlorogenic acid and caffeine is exposed in coffee, thymol and carvacrol in oregano. Three different oils were used to prepare organogels (coconut, olive and hemp) and 2 different organogelators (stearic acid and ethyl cellulose). In the case of using hemp and olive oil and gelators SA and EC we found out, there were no problems in the preparation of organogels, while in use of coconut oil and gelator EC we did not get a suitable organogel. When formulating coffee extract into organogels, we found out that only formulations with EC gelled unevenly, otherwise clusters of gels were formed. When formulating oregano extract into organogels, our formulations were inadequate, the exception being the organogel prepared from hemp oil and EC, which contained 10% of the extract. We also carried out the formulation of pure components into organogels. In the case of thymol,organogel formulations prepared from olive oil successfully gelled. We also obtained successfully gelled organogels with coconut oil and hemp oil, with the exception of one formulation with each oil. When formulating carvacrol, the organogel formulations prepared from hemp oil gelled successfully, while when using coconut and olive oil, the gelation in one sample of both formulations was not satisfactory. Finally, when formulated chlorogenic acid into an organogenic pure component, the adequate gelation was obtained for all formulations. When checking the rheological properties, we found that the viscosity curves almost matched the behavior of the flow curves for our organogels, with a general decrease in viscosity with increasing shear rate. As a final step, we dissolved various formulations of the prepared organogels in 0.1 M HCl. The samples were analyzed using LC-MS/MS and GC-MS. We found out, that chlorogenic acid achieved the best results in the release of various active ingredients, and suitable content curves for caffeine and chlorogenic acid were also obtained by dissolving the organogel formulations with coffee extract. The use of other formulations did not show adequate results. zelena kava origano organogeli viskoznost raztapljanje dostavnih sistemov green coffee oregano organogels viscosity dissolution delivery systems true true false [M. Ostroško] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2023-03-06 13:59:13 2023-03-23 13:22:36 2023-04-04 03:05:02 0000-00-00 00:00:00 2023 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (XI, 70 f.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.28.015:633.88(043.2) 147497987 MAG_Ostrosko_Monika_2023.pdf MAG_Ostrosko_Monika_2023.pdf 1 D5EB9BD1A2B5FF91AA3B3B18F4169F54 77690a62b3ab5c4d8b71310244c7bccab920cd9bc08a64f59c1e4ddb19afac1a 5448cdaa-c96e-11ed-98fa-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=166255 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0