20.500.12556/DKUM-84160 Functional 3D printed polysaccharide derivative scaffolds for vascular graft application doctoral disertation Funkcionalni 3D tiskani celični nosilci iz polisaharidnih derivatov za vaskularne vsadke Tissue engineering (TE) is an interdisciplinary field that aims towards replacement, healing or reconstruction of damaged tissue and organs. Incurable diseases are currently treated with organ transplantation, that have the disadvantages of insufficient donors, immune response, and organ rejection after transplantation. TE imitate the functions of extracellular matrix (ECM) to develop biocompatible/biodegradable scaffolds with appropriate features which are utilized to provide mechanical support, cellular infiltration, migration, and tissue formation, and to mimic the biochemical and biophysical cues of cells. Several fabrication methods have been introduced to mimic the 3D structure of ECM and 3D printing is one of the additive manufacturing techniques, widely used in TE because of its feasibility to build complex tissue constructs and control over fabrication and cell distribution. The polysaccharide-peptide conjugate has gained enormous interest in recent years owing to its biocompatibility, degradability, flexibility, and structural matching to natural proteoglycans. In this context, we reported here on investigation of biocompatibility with HUVECs, surface modification of 3D printed PCL scaffolds with an amine group and chemically crosslinked oxidized HA-amino acid/peptide conjugates (OHACs) was used to develop a novel biomaterial for use as a tissue engineered vascular graft. Modified polysaccharides were characterized with respect to their chemical structure, charge, UV and fluorescence properties and cytotoxicity. The successful conjugation was demonstrated by XPS, and a decrease in the free amine peaks on the surface was observed after conjugation. In addition, the water contact angle measurements showed improved wetting, an indication that the conjugation to the PCL-A surface was successful. Finally, the biocompatibility of the novel scaffolds was characterized by the MTS and the live- dead assay. In both assays, proliferation of cells was observed after 7 days and cell spreading on the surface was detected by phalloidin staining of actin filaments. In conclusion, it was possible to prepare surface-active scaffolds by combining the advantages of biocompatibility and mechanical strength of polysaccharides and polyesters, respectively. Tkivno inženirstvo (TE) je interdisciplinarno področje, katerega cilj je nadomestitev, zdravljenje ali rekonstrukcija poškodovanih tkiv in organov. Neozdravljive bolezni se trenutno zdravijo s presaditvijo organov, ki pa imajo pomanjkljivosti, kot so nezadostno število darovalcev, imunski odziv in zavrnitev organa po presaditvi. TE posnema funkcije zunajceličnega matriksa (ECM), da bi razvili biokompatibilne/biorazgradljive skelete z ustreznimi lastnostmi, ki se uporabljajo za zagotavljanje mehanske podpore, infiltracijo celic, migracijo in tvorbo tkiva ter posnemanje biokemičnih in biofizikalnih znakov celic. Uvedenih je bilo več metod izdelave za posnemanje 3D-strukture ECM, 3D-tiskanje pa je ena od tehnik aditivne proizvodnje, ki se pogosto uporablja v TE zaradi možnosti izdelave kompleksnih tkivnih konstrukcij ter nadzora nad izdelavo in porazdelitvijo celic. Konjugat polisaharid-peptid je v zadnjih letih zaradi svoje biokompatibilnosti, razgradljivosti, prožnosti in strukturne skladnosti z naravnimi proteoglikani požel ogromno zanimanja. Na podlagi tega poročamo o raziskavi biokompatibilnosti s HUVEC, površinski modifikaciji 3D tiskanih PCL-skladov z aminsko skupino in kemično zamreženih oksidiranih konjugatov HA-amino kislin/peptidov (OHAC) za razvoj novega biomateriala za uporabo kot žilni presadek za tkivno inženirstvo. Modificirani polisaharidi so bili karakterizirani glede na kemijsko strukturo, naboj, UV in fluorescenčne lastnosti ter citotoksičnost. Uspešna konjugacija je bila dokazana z metodo XPS, po konjugaciji pa je bilo opaziti zmanjšanje prostih aminskih vrhov na površini. Poleg tega so meritve kontaktnega kota vode pokazale izboljšano omočljivost, kar kaže, da je bila konjugacija na površino PCL-A uspešna. Na koncu je bila biokompatibilnost novih ogrodij opredeljena s testom MTS in «live-dead« testom. V obeh testih je bila po 7 dneh opažena proliferacija celic, širjenje celic na površini pa je bilo ugotovljeno z barvanjem aktinskih filamentov s falloidinom. Zaključimo lahko, da je bilo mogoče pripraviti površinsko aktivne skelete z združitvijo prednosti biokompatibilnosti in mehanske trdnosti polisaharidov oziroma poliestrov. 3D tiskanje karboksimetilceluloza hialuronska kislina polikaprolakton kemija karbodiimida kemija Shiffove baze endotelizacija 3D printing carboxymethyl cellulose hyaluronic acid polycaprolactone carbodiimide chemistry shiff-base chemistry endothelialization 3D tiskanje karboksimetilceluloza hialuronska kislina polikaprolakton kemija karbodiimida kemija Shiffove baze endotelizacija true true false [F. Gürer] Angleški jezik Slovenski jezik Doktorsko delo/naloga 2023-04-21 13:40:55 2023-10-06 09:34:38 2023-10-26 03:35:42 0000-00-00 00:00:00 2023 0 Maribor 0 Maribor XXII, 138 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 [604.2:547.458]:616-003.93(043.3) 169307139 Fazilet_Gurer__Doctoral_dissertation_Final.pdf Fazilet_Gurer__Doctoral_dissertation_Final.pdf 1 5E07EEBDCB9D96D41F417BB367507D62 151ae4b3082eec68ec98fc4fa81921a830643765ebcfb93878ca0f8ec86b7e0f a26922ff-730e-11ee-934d-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=173752 Fakulteta za strojništvo 0 0 0