20.500.12556/DKUM-85074 Ekstrakcija olj iz semen industrijske konoplje in karakterizacija biološko aktivnih komponent magistrsko delo Hemp seeds extraction and characterisation of biological component Konopljino olje je zelo cenjen proizvod, saj vsebuje esencialne aminokisline in visoke deleže (več kot 80%) polinenasičenih maščobnih kislin (PUFA). Razmerje γ-linolenske kisline (omega 6) in α -linolenske kisline (omega 3) v konopljinem olju se giblje med 2,1 in 3,1. Poleg tega konopljino olje vsebuje tudi stearinsko kislino, terpenoide in druge biološko zanimive komponente. Iz konopljinih semen smo s pomočjo konvencionalnih metod (ekstrakcija po Soxhletu, hladna maceracija, hladno stiskanje) in nekonvencionalnih metod (ekstrakcija z ultrazvokom, superkritična ekstrakcija) pridobili različne ekstrakte in jim določili totalne fenole, antioksidativni potencial in vsebnost maščobnih kislin z GC-MS. Predvsem smo se posvetili omega 3 in omega 6 maščobnim kislinam, kjer nas je zanimala njihova vsebnost v ekstraktih in organogelih. Hkrati smo primerjali sestavo maščobnih kislih svežih ter staranih ekstraktov in organogelov. Rezultati kažejo, da smo uspešno izolirali želene komponente iz konopljinega semena. Ekstrakti in organogeli so vsebovali od 48 do 70% maščobnih kislin. Razmerja med linolno in linolensko kislino pa so bila med 2,65 in 2,8. S tvorjenjem organogelov pa smo uspešno zamrežili maščobne kisline in upočasnili njihovo razpadanje ter dosegli kontrolirano hitrost sproščanja. Hemp oil is a highly valued product, as it contains essential amino acids and high proportions (more than 80%) of polyunsaturated fatty acids (PUFA). The ratio of γ-linolenic acid (ω-6) and α-linolenic acid (ω-3) in hemp oil ranges from 2.1 to 3.1. In addition, hemp oil also contains stearic acid, terpenoids and other biologically interesting components. Using conventional methods (ultrasound extraction, Soxhlet extraction, cold maceration), non-conventional methods (supercritical extraction, cold-pressed oil), we are obtain various extracts from hemp seeds and determine their total phenols, antioxidant activity and the content of various fatty acids by GC-MS. In particular, we are focus on omega 3 and omega 6 fatty acids, where we are interested in their content in extracts and organogels. At the same time, we compare the composition of fresh and aged fatty acid extracts and organogels. The results show that we have successfully isolated biocomponents from hemp seeds. Extracts and organogels contained from 48 to 70% fatty acids. The ratios between linoleic and linolenic acids ranged between 2.65 and 2.8. By forming organogels, fatty acids were successfully cross-linked and their decomposition and solubility rate has been controlled konopljina semena konopljino olje organogel maščobne kisline totalni fenoli antioksidativna aktivnost hemp seeds hemp oil organogel fatty acids total phenols antioxidant activity true true false [S. Karlovšek] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2023-08-17 15:14:54 2023-09-06 10:04:07 2023-09-28 03:05:36 0000-00-00 00:00:00 2023 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (XI, 49 f.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2026-09-01 543.635.3:633.522(043.2) 165927939 MAG_Karlovsek_Sara_2023.pdf MAG_Karlovsek_Sara_2023.pdf 1 F52E7A9685566F691DC18C5B8ADDFFFC 8647c4abb9fa9e34e1f22494f9d168c06ed91815c4ae8f667ab10f272d4723e4 05b839f6-4c02-11ee-9b29-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=171530 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0