20.500.12556/DKUM-85106 Amoxicillin doped hyaluronic acid/fucoidan multifunctional coatings for medical grade stainless steel orthopedic implants This work investigated the potential of amoxicillin-doped hyaluronic acid/fucoidan multifunctional coatings on medical grade stainless steel as biocompatible, osteointegration enhancing, antimicrobial, and bacterial biofilm inhibiting coatings for orthopedic implants. The coatings were prepared by layer-by-layer spin coating and confirmed by optical contact angle goniometry and infrared spectroscopy. Time-of-flight secondary ion mass spectrometry showed a homogeneous distribution of the individual layers. In contrast, thermal diffusivity, and thermal conductivity measurements by photothermal beam deflection spectrometry showed diffusion of the amoxicillin into the hyaluronic acid and fucoidan layers. In vitro release of amoxicillin showed complete release within one hour, as reflected by the formation of inhibition zones against Staphylococcus aureus and Escherichia coli. Synergistic effects were observed between the hyaluronic acid and amoxicillin in inhibiting S.Aureus biofilm, and between the fucoidan and amoxicillin in improving the antioxidant properties by an ABTS radical scavenging assay. Biocompatibility was determined with human osteoblasts, confirming the potential of such multifunc- tional coatings to enhance the bioactivity of steel-based orthop edic implants. orthopedic implant medical grade stainless steel amoxicillin hyaluronic acid fucoidan multifunctional coating ortopedski vsadek medicinsko nerjaveče jeklo amoksicilin hialuronska kislina fukoidan večnamenska premaz true false true Elsevier Angleški jezik Slovenski jezik https://www.sciencedirect.com/journal/applied-surface-science Članek v reviji 2023-08-18 13:12:19 2023-08-18 13:12:19 2024-04-16 15:07:08 0000-00-00 00:00:00 2023 0 0 str. 1-11 part A Letn. 611 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-07-19 2022-11-04 2022-11-09 54 130119171 10.1016/j.apsusc.2022.155621 0169-4332 Amoxicillin_doped_hyaluronic_acid_-Bracic-2023.pdf Amoxicillin_doped_hyaluronic_acid_-Bracic-2023.pdf 1 375B2C77A8351DBC9927D591F55B1D5D 426c6cbd03133c0a891df066a35b3bf854b464004c8b221a6123e6250c34b86c 55775635-3db9-11ee-8efa-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=169765 https://www.sciencedirect.com/science/article/pii/S016943322203149X?via%3Dihub 0 5d398aa7-3db9-11ee-8efa-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=169766 Fakulteta za strojništvo Fakulteta za kemijo in kemijsko tehnologijo Medicinska fakulteta 0 0 0