20.500.12556/DKUM-85297 Razvoj materialov z aktivnim kisikom za hitrejše celjenje ran magistrsko delo Development of oxygen-releasing materials for wound healing Magistrsko delo predstavlja sintezo, razvoj in karakterizacijo do sedaj še neobstoječih biorazgradljivih materialov - aerogelov z aktivnim kisikom za namene hitrejšega celjenja ran. Kot vhodna materiala smo uporabili polisaharid ksantan in biopolimer polilaktično kislino (PLA), ki sta biokompatibilna in cenovno dostopna. Ksantan služi kot osnovni material, ki enkapsulira aktivne učinkovine za namene kontroliranega sproščanja, medtem ko PLA izboljša mehanske lastnosti našega materiala. V sklopu našega raziskovalnega dela smo po sol-gel postopku s kombiniranjem PLA v etil laktatu ter ksantana v vodi pripravili kompozitne hidrogele. S superkritičnim sušenjem smo jih pretvorili v aerogele in okarakterizirali s plinsko adsorpcijo. S tem smo določili velikost specifičnih površin (BET), ki so dosegle vrednosti do 396 m2/g, ter povprečno velikosto in prostornino por v mezoporoznem območju. Izvedli smo tudi termično analizo z DSC/TGA, test nabrekanja in stabilnosti ter z njimi ocenili termično stabilnost in lastnosti razpada materialov. Rezultati so pokazali, da je kombinacija ksantana in PLA podaljšala stabilnost materiala v simulirani telesni tekočini (SBF) tudi do 72 ur. Materiali z večjo vsebnostjo PLA so absorbirali SBF do 67 kratnika svoje mase, kar je najverjetneje posledica boljše stabilnosti v SBF. Sestavo aerogelov in s tem prisotnost posameznih komponent smo potrdili z analizo FT IR. Glede na rezultate karakterizacije smo izbrali dva materiala z najobetavnejšimi lastnostmi, ter v njiju vključili učinkovini, ki ob stiku z rano generirata kisik, ter zdravilno učinkovino deksametazon. Sproščanje kisika iz materiala smo določili s standardno metodo izpodrivanja vode, pri kateri je bila količina izpodrinjene vode neposredno sorazmerna količini nastalega kisika. Meritve smo izvajali 48 ur, rezultati pa so pokazali, da je material sproščal kisik v celotnem časovnem intervalu. Testirali smo tudi sproščanje deksametazona v SBF, ki je bilo uspešno v primeru obeh impregniranih aerogelov. Nazadnje smo preverili antimikrobno delovanje materialov proti bakterijam Escherichia coli in Staphylococcus aureus. Oba aerogela sta inhibirala rast bakterij in s tem izkazala antimikrobno učinkovitost. This master thesis presents the first synthesis, development, and characterization of biodegradable materials - aerogels with active oxygen for faster wound healing. The natural polysaccharide xanthan gum and the biopolymer polylactic acid (PLA) were selected as precursor materials for the preparation of aerogels. Xanthan gum served as a matrix material that encapsulated the active ingredients for controlled-release, while PLA improved the mechanical properties of our material. As part of our research, we prepared hybrid hydrogels by a sol-gel process combining PLA in ethyl lactate and xanthan gum in water. They were converted into aerogels by supercritical drying and characterized by gas adsorption. This was used to determine their specific surface areas (BET), which reached values of up to 396 m2/g, and to determine the average pore size and pore volume in the mesoporous region. Thermal analysis with DSC/TGA, swelling, and stability tests were also performed to evaluate the thermal stability and structural integrity of aerogels. The results showed that the combination of xanthan gum and PLA extended the stability of the material in simulated body fluid (SBF) for up to 72 hours. Materials with higher PLA content absorbed up to 67 times their weight and showed a very high fluid absorption capacity, likely due to better stability in SBF. The composition of the aerogels and thus the presence of individual components was confirmed by FT-IR analysis. Based on the characterization results, we selected two materials with the most promising properties, added oxygen-generating agents, and impregnated them with dexamethasone. Oxygen release from the materials was determined by the standard water displacement method, and the amount of water displaced was directly proportional to the amount of oxygen produced. Measurements were performed for 48 hours, and the results showed that the materials released oxygen throughout the period. The drug release profile in SBF was also determined and showed that impregnation was successful for both aerogels. Finally, the antimicrobial activity of the materials was tested against bacteria Esherichia coli and Staphylococcus aureus. Both aerogels successfully inhibited bacterial growth, demonstrating antimicrobial activity. aerogel aktivni kisik biomaterial celjenje kroničnih ran kontrolirano sproščanje aerogel active oxygen biomaterial chronic wound healing controlled release true true false [N. Atelšek Hozjan] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2023-08-24 18:46:21 2023-09-18 10:38:01 2023-10-28 03:16:40 0000-00-00 00:00:00 2023 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (XI, 67 f.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 544.774.2:[604.4:615.33](043.2) 170247939 MAG_Atelsek_Hozjan_Nika_2023.pdf MAG_Atelsek_Hozjan_Nika_2023.pdf 1 FAB674DA262E31DF4E14976E115987F0 55af79bbc159895f254e997bc811041a82bc32ff5ce3a10bea9e95b167c02437 0786bd7a-53ba-11ee-8ecf-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=172333 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0