20.500.12556/DKUM-85461 Optical detection of stress parameters diploma thesis Optična detekcija stresnih parametrov In this thesis we developed the optical method of dopamine detection at pH 9, by creating DA-OPA complex. O–phthaldehyde was used as the indicator for fluorescent detection of dopamine with (3-Mercaptopropyl)-triethoxysilane as an additional reagent. The fluorescent product reached its maximum after 2 minutes, wavelengths of emission and excitation were λex/λem=340 nm/460 nm. The determination of dopamine was linear within a concentration range between 0.5∙10–6 and 3∙10–6 M. Limit of detection was 3.38∙10–6 M. Urea and norepinephrine can interfere dopamine detection, under examined parameters: ratio DA:OPA was 1:100, OPA:MPTES was 1:1, buffer was at pH 9. V diplomskem delu smo razvili optično metodo za detekcijo dopamina pri pH 9, s tvorbo kompleksa DA-OPA. O-ftaldehid smo uporabili kot indikator za fluorescenčno detekcijo dopamina ter (3-Merkaptopropil)-trietoksisilan kot dodaten reagent. Fluorescenčni produkt je dosegel svoj maksimum po 2 minutah, valovne dolžine izsevanja in vzbujanja so bile λex/λem=340 nm/460nm. Določanje dopamine je bilo linearno v koncentracijskem območju med 0,5∙10–6 and 3∙10–6 M. Meja detekcije je znašala 3,38∙10–6 M. Urea in norepinefrin lahko vplivata na detekcijo dopamina pod določenimi parametri: razmerje DA:OPA je 1:100, OPA:MPTES je 1:1, pufer ima pH 9. dopamine fluorescence o-phthaldehyde optical method dopamin fluorescenca o-ftaldehid optična metoda true true false Angleški jezik Slovenski jezik Diplomsko delo/naloga 2023-08-30 12:35:59 2023-09-13 07:32:22 2023-09-23 03:08:23 0000-00-00 00:00:00 2023 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (VIII, 36 f.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.426:577.17(043.2) 165411075 UN_Krstic_Marijana_2023.pdf UN_Krstic_Marijana_2023.pdf 1 1560087EF4D29BD493C2A7D92DA618ED 1c39dbe583bcc82329f69c4e43ec01554f4e1fa725caf972e4e38ea61f0c485e a21cfc5e-5091-11ee-a242-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=171958 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0