20.500.12556/DKUM-85737 Pullulan-based films impregnated with silver nanoparticles from the Fusarium culmorum strain JTW1 for potential applications in the food industry and medicine Introduction: Biopolymers, such as pullulan, a natural exopolysaccharide from Aureobasidium pullulans, and their nanocomposites are commonly used in the food, pharmaceutical, and medical industries due to their unique physical and chemical properties. Methods: Pullulan was synthesized by the A. pullulans ATCC 201253 strain. Nanocomposite films based on biosynthesized pullulan were prepared and loaded with different concentrations of silver nanoparticles (AgNPs) synthesized by the Fusarium culmorum strain JTW1. AgNPs were characterized by transmission electron microscopy, Zeta potential measurements, and Fourier-transform infrared spectroscopy. In turn, the produced films were subjected to physico-chemical analyses such as goniometry, UV shielding capacity, attenuated total reflection–Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy, and their mechanical and degradation properties were assessed. The antibacterial assays of the nanoparticles and the nanocomposite films against both food-borne and reference pathogens, including Listeria monocytogenes, Salmonella infantis, Salmonella enterica, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae, were performed using standard methods. Results: AgNPs were small (mean 15.1 nm), spherical, and displayed good stability, being coated with protein biomolecules. When used in higher concentrations as an additive to pullulan films, they resulted in reduced hydrophilicity and light transmission for both UV-B and UV-A lights. Moreover, the produced films exhibited a smooth surface. Therefore, it can be concluded that the addition of biogenic AgNPs did not change the morphology and texture of the films compared to the control film. The nanoparticles and nanocomposite films demonstrated remarkable antibacterial activity against both food-borne and reference bacteria. The highest activity of the prepared films was observed against L. monocytogenes. Discussion: The obtained results suggest that the novel nanocomposite films prepared from biosynthesized pullulan and AgNPs can be considered for use in the development of medical products and food packaging. Moreover, this is the first report on pullulan-based nanocomposites with mycogenic AgNPs for such applications. Aureobasidium pullulans pullulan nanocomposite films silver nanoparticles mycosynthesis nanobiotechnology applied microbiology antibacterial activity nanotehnologija nanodelci ikrobiologija antibakterijske lastnosti true true true Frontiers Media Angleški jezik Slovenski jezik https://www.frontiersin.org/journals/bioengineering-and-biotechnology Copyright © 2023 Wypij, Rai, Zemljič, Bračič, Hribernik and Golińska. Članek v reviji 2023-09-08 10:10:07 2023-09-08 10:10:07 2024-06-04 08:16:03 0000-00-00 00:00:00 2023 0 0 17 str. Vol. 11 Avg. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2023-06-17 2023-07-25 2023-08-07 620.3 162939907 10.3389/fbioe.2023.1241739 2296-4185 fbioe-11-1241739.pdf fbioe-11-1241739.pdf 1 265A50289735305328B2EAA37CE22516 9991f222d3d33e198860a872591213c1a99162d97f2bc8506c1a6750d3b1930c 447aa41e-4e1f-11ee-9575-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=171806 https://www.frontiersin.org/articles/10.3389/fbioe.2023.1241739/full 0 5798bab5-4e20-11ee-9575-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=171808 Fakulteta za strojništvo 0 0 0