20.500.12556/DKUM-85884 Vpliv izhodiščnega materiala in različnih načinov razkuževanja na vzpostavitev tkivne kulture jagodnjaka ( fragaria x ananassa duchesne) diplomsko delo Influence of starting material and different disinfection methods on strawberry ( fragaria ? duchesne) tissue culture establishment V letu 2023 je bil na Fakulteti za kmetijstvo in biosistemske vede v Mariboru izveden poskus, v katerem se je ugotavljalo kateri način razkuževanja rastlinskega materiala je najbolj optimalen za kasnejšo uspešno vegetativno razmnoževanje jagodnjaka, Fragaria × ananassa Duchesne v in vitro razmerah. Drug namen raziskave je bila primerjava uspešnosti razkuževanja glede na izvor nabranega materiala (rastline, ki rastejo v zaščitenem prostoru v primerjavi s tistimi, ki rastejo na prostem). Za razkuževanje sta bili uporabljeni natrijev hipoklorit (NaClO) in dikloroizocianurna kislina (DICA) v različnih koncentracijah ter časih tretiranja, skupaj 4 različna obravnavanja. Razkuženi izsečki so bili nameščeni na osnovno MS gojišče brez dodanih rastnih regulatorjev, po treh tednih so bili vsi izsečki prestavljeni na gojišče, kateremu je bil dodan antibiotik Cefotaxime sodium. Izkazalo se je, da je to preprečilo propad tistih rastlin, ki so že pokazale potencial in začele z rastjo, vendar je bilo tekom inokulacije zaznati bakterijske okužbe. V raziskavi je bilo ugotovljeno, da tako način razkuževanja, kot izvor rastlinskega materiala vplivata na število dobljenih vitalnih poganjkov. Pri izsečkih jagodnjaka iz zaščitenega prostora je V bil uspeh razkuževanja 13,3 % oz. 16 izsečkov, iz nezaščitenega prostora pa 3,3 % oz. 4 izsečki. Med različnimi obravnavanji se je kot najbolj uspešno izkazalo razkuževanje s sredstvom DICA in časom tretiranja 1,5 min, kjer je bil uspeh 3,75 % (9 vitalnih izsečkov). Pri uporabi 1,5 % NaClO in 1,5 min, je bil uspeh 2,08 % oz. 5 vitalnih izsečkov. Pri ostalih dveh obravnavanjih je bil uspeh 1,25 %. An experiment was conducted in 2023 at the Faculty of Agriculture and Life Sciences in Maribor, that determined which method of disinfection of plant material is the most suitable for the subsequent successful vegetative propagation of strawberry, Fragaria × ananassa Duchesne under in vitro conditions. The purpose was also to compare the effectiveness of disinfection depending on the origin of the collected material (plants growing in a protected area compared to those growing outdoors). Sodium hypochlorite (NaClO) and dichloroisocyanuric acid (DICA) were used for disinfection at different concentrations and treatment times for a total of 4 different treatments. Disinfected explants were placed on a basic MS breeding ground without added growth regulators, and after three weeks all cuttings were transferred to a breeding ground to which the antibiotic Cefotaxime sodium was added. It was found that this prevented the collapse of those plants that had already shown potential and started to grow, however, during inoculation bacterial infections were detected. The study shows that both the disinfection method and the origin of the plant material influence the number of VII obtained viable shoots. For strawberry cuttings from the protected area, disinfection success was 13.3% or 16 explants, while from the unprotected area 3.3% or 4 explants. Among the different treatments, disinfection with DICA and a treatment time of 1.5 min proved to be the most successful, with a success rate of 3.75% (9 vital shoots). For the treatment with 1.5% NaClO and 1.5 min, the success rate was 2.08% or 5 vital shoots. For the other two treatments, the success rate was 1.25%. jagodnjak Fragaria × ananassa in vitro razkuževanje tkivne kulture strawberry Fragaria × ananassa in vitro desinfection tissue culture true true false S. Lešnik Slovenski jezik Angleški jezik Diplomsko delo/naloga 2023-09-17 21:27:15 2023-10-11 13:47:00 2023-10-12 03:07:10 0000-00-00 00:00:00 2023 0 Maribor 0 Maribor 1 spletni vir (1 PDF datoteka (XIII, 37 str., [4] str. pril.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.75:631.532/.533:631.53.027.2(043.2)=163.6 167967491 VS_Lesnik_Sergeja_2023.pdf VS_Lesnik_Sergeja_2023.pdf 1 9A7E73FC4FAD8E167E97C272757616A0 d1d71ab573e010789332e8e43afec06aa39b5c14f0b90547688e82309e0cb278 82b80f50-585a-11ee-a4d2-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=172727 Fakulteta za kmetijstvo in biosistemske vede 0 0 0