20.500.12556/DKUM-86411 Efficient and Green Isolation of Keratin from Poultry Feathers by Subcritical Water The isolation of keratin from poultry feathers using subcritical water was studied in a batch reactor at temperatures (120–250 °C) and reaction times (5–75 min). The hydrolyzed product was characterized by FTIR and elemental analysis, while the molecular weight of the isolated product was determined by SDS-PAGE electrophoresis. To determine whether disulfide bond cleavage was followed by depolymerization of protein molecules to amino acids, the concentration of 27 amino acids in the hydrolysate was analyzed by GC/MS. The optimal operating parameters for obtaining a high molecular weight protein hydrolysate from poultry feathers were 180 °C and 60 min. The molecular weight of the protein hydrolysate obtained under optimal conditions ranged from 4.5 to 12 kDa, and the content of amino acids in the dried product was low (2.53% w/w). Elemental and FTIR analyses of unprocessed feathers and dried hydrolysate obtained under optimal conditions showed no significant differences in protein content and structure. Obtained hydrolysate is a colloidal solution with a tendency for particle agglomeration. Finally, a positive influence on skin fibroblast viability was observed for the hydrolysate obtained under optimal processing conditions for concentrations below 6.25 mg/mL, which makes the product interesting for various biomedical applications. poultry feathers subcritical water hydrolysis keratin physico-chemical characterization cytotoxicity subkritična hidroliza vode fizikalno kemijske lastnosti citotoksičnost true true true MDPI Angleški jezik Slovenski jezik https://www.mdpi.com/ © 2023 by the authors Članek v reviji 2023-11-28 12:20:33 2023-11-28 13:40:34 2024-04-10 11:01:29 0000-00-00 00:00:00 2023 0 0 str. 2658 no. 12 Vol. 15 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2023-05-16 2023-06-09 2023-06-12 66.02 2073-4360 155799299 10.3390/polym15122658 2073-4360 polymers-15-02658-v2.pdf polymers-15-02658-v2.pdf 1 ADC560177A388899C11397A9713070BF c241fc326e5ee6314f2f7d1d0652d091b95c4a6d7a9068923125af896c380237 f092c510-8de2-11ee-b06a-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=174381 https://www.mdpi.com/2073-4360/15/12/2658 0 eb23189f-f718-11ee-957c-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=178193 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0