20.500.12556/DKUM-86524 Study of gold nanoparticles conjugated with SARS-CoV-2 S1 spike protein fragments This study reports on the successful conjugation of SARS-CoV-2 S1 spike protein fragments with gold nanoparticles (AuNPs) that were synthesised with Ultrasonic Spray Pyrolysis (USP). This method enables the continuous synthesis of AuNPs with a high degree of purity, round shapes, and the formation of a surface that allows various modifications. The conjugation mechanism of USP synthesized AuNPs with SARS-CoV-2 S1 spike protein fragments was investigated. A gel electrophoresis experiment confirmed the successful conjugation of AuNPs with SARS-CoV-2 S1 fragments indirectly. X-ray Photoelectron Spectroscopy (XPS) analysis confirmed the presence of characteristic O1s and N1s peaks, which indicated that specific binding between AuNPs and SARS-CoV-2 S1 spike protein fragments takes place via a peptide bond formed with the citrate stabiliser. This bond is coordinated to the AuNP’s surface and the N-terminals of the protein, with the conjugate displaying the expected response within a prototype LFIA test. This study will help in better understanding the behaviour of AuNPs synthesised with USP and their potential use as sensors in colorimetric or electrochemical sensors and LFIA tests. gold nanoparticles ultrasonic spray pyrolysis conjugation SARS-CoV-2 S1 characterisation nanodelci zlata ultrazvočna razpršilna piroliza konjugacija SARS-CoV-2 S1 karakterizacija true false true MDPI https://www.mdpi.com/2079-4991/13/15/2160 Angleški jezik Slovenski jezik https://www.mdpi.com/ Članek v reviji 2023-12-07 09:31:16 2023-12-07 09:31:17 2024-04-04 09:43:04 0000-00-00 00:00:00 2023 0 0 str. 1-14 iss. 15, [article no.] 2160 Vol. 13 Aug. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2023-06-29 2023-07-24 2023-07-25 669.2:620.3 159776515 10.3390/nano13152160 2079-4991 nanomaterials-13-02160-v2_(1).pdf nanomaterials-13-02160-v2_(1).pdf 1 A441F9DB4632ECD180BFE7794B045175 28c20a627cb1b01e30e80fb26bcd9af2d6352246d4ab02d75fe7b675f6e81add 71191221-95ab-11ee-a137-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=174753 https://www.mdpi.com/2079-4991/13/15/2160 0 b1e51c94-95ab-11ee-a137-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=174754 Fakulteta za strojništvo 0 0 0