20.500.12556/DKUM-86559 Vpliv različnih načinov sterilizacije in tipa brstov na uspešno vzpostavitev tkivne kulture pri navadni bodiki ( Ilex aquifolium L.) diplomsko delo Effect of different sterilisation techniques and common holly ( Ilex aquifilium l.) bud type on the success of establishing tissue culture V letu 2023 smo na Fakulteti za kmetijstvo in biosistemske vede v Mariboru opravili poskus, v katerem smo proučevali vpliv različnih načinov sterilizacije in vpliv tipa brstov (apikalni in aksilarni) na vzpostavitev tkivne kulture navadne bodike (Ilex aquifolium L.). Za razkuževanje smo uporabili dve različni sterilizacijski sredstvi, dikloroizocianurno kislino (DICA) in natrijev hipoklorit (NaClO), v različnih koncentracijah in z različnim časom tretiranja. V raziskavo je bilo vključenih 640 brstov, na katerih smo skupno opravili 7 različnih načinov sterilizacije. Vse brste smo predhodno tretirali v 70-% etanolu za 40 sekund. Razkužene brste smo prenesli na osnovno gojišče McCown Woody Plant in spremljali rast. Ugotovili smo, da tako način sterilizacije kot tudi tip brstov vplivata na pridobljeno število vitalnih poganjkov. Skupno smo uspešno sterilizirali 22 brstov. Za uspešnejše so se izkazali apikalni brsti. Pri primerjavi sterilizacijskih sredstev se je tretiranje z DICA izkazalo za uspešnejše, pridobili smo 18 vitalnih brstov. Najuspešnejša je bila sterilizacija brstov z DICA in časom tretiranja 5 minut, kjer smo pridobili 7 vitalnih brstov. In 2023, an experiment was conducted at the Faculty of Agriculture and Life Sciences in Maribor, to investigate the effect of different sterilisation techniques and the influence of bud type (apical and axillary) on the establishment of tissue culture of common holly (Ilex aquifolium L.). Two different sterilising agents, dichloroisocyanuric acid (DICA) and sodium hypochlorite (NaClO), were used in different concentrations and treatment times for disinfection. The study included 640 buds and seven sterilisation treatments. All buds were first treated in 70 % ethanol for 40 seconds. After disinfection, the buds were placed on McCown Woody Plant Medium and their growth was observed. The results showed that both the method of sterilisation and the type of buds influenced the number of viable shoots obtained. Of the 640 buds, only 22 were successfully sterilised, with the apical buds proved to be more successful. When comparing the sterilisation methods, the DICA treatment proved to be more successful, as 18 viable buds were obtained. The most effective sterilization method was a 5-minute treatment of the buds with DICA, which produced seven viable buds. sterilizacija tkivne kulture bodika in vitro brsti sterilisation tissue culture holly in vitro buds true true false U. Modrič Slovenski jezik Angleški jezik Diplomsko delo/naloga 2023-12-12 22:12:18 2024-01-08 14:23:37 2024-01-09 03:10:45 0000-00-00 00:00:00 2023 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (IX, 10-45, [1] str.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 631.533.3:582.314.1:57.086.83(043.2)=163.6 179955971 VS_Modric_Urska_2023.pdf VS_Modric_Urska_2023.pdf 1 17A45231802998FB39CF51B388F33A88 82dd8a4b3cb8449c036a6d0061b796006aa82f2884b98bd2f283dbfe6e365425 c2d0d68a-a5e2-11ee-8081-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=175046 Fakulteta za kmetijstvo in biosistemske vede 0 0 0