20.500.12556/DKUM-86944 Polyvinylpyrrolidone-stabilised gold nanoparticle coatings inhibit blood protein adsorption In this work, the ability of polyvinylpyrrolidone (PVP)-stabilised gold nanoparticle (AuNP) coatings to inhibit blood protein adsorption was evaluated by studying timeresolved solid–liquid interactions of the coatings with the model blood protein bovine serum albumin (BSA). Inhibiting unspecific blood protein adsorption is of crucial importance for blood-contacting implant devices, e.g. vascular grafts, stents, artificial joints, and others, as a preventive strategy for bacterial biofilm formation. A quartz crystal microbalance was used in this work to coat the AuNPs on piezoelectric sensors and to follow time-resolved solid–liquid interactions with the proteins. The AuNP coatings were evaluated for their wettability by contact angle measurements, their surface morphology by lightand atomic force microscopy, and their chemical composition by energy-dispersive X-ray spectroscopy. Results revealed a homogeneous distribution of AuNPs on the sensor surface with a dry mass coverage of 3.37 ± 1.46 µg/cm2 and a contact angle of 25.2 ± 1.1°. Solid–liquid interaction studies by quartz crystal microbalance showed a high repellence of BSA from the PVP-stabilised AuNP coatings and the importance of the PVP in the mechanism of repellence. Furthermore, the conformation of the polymer on the coatings as well as its viscoelastic properties were revealed. Finally, the activated partial thrombin time test and fibrinogen adsorption studies revealed that the AuNPs do not accelerate blood coagulation and can partially inhibit the adhesion of fibrinogen, which is a crucial factor in the common blood coagulation cascade. Such AuNPs have the potential to be used in blood-contact medical applications. gold nanoparticles ultrasonic spray pyrolysis haemocompatibility protein adsorption quartz crystal microbalance zlati nanodelci ultrazvočna razpršilna piroliza hemokompatibilnost adsorpcija proteina kremenova mikrotehtnica true false true De Gruyter Angleški jezik Slovenski jezik https://www.degruyterbrill.com/journal/key/ntrev/html © 2024 the author(s) Članek v reviji 2024-01-30 16:12:01 2024-01-30 16:12:06 2025-07-01 09:14:11 0000-00-00 00:00:00 2024 0 0 13 str. iss. 1 Vol. 13 Jan. 2024 0000-00-00 Zaloznikova Objavljeno apc 2023-10-02 2023-12-04 2024-01-20 669.2/.8:620.3 182195459 10.1515/ntrev-2023-0176 2191-9097 10.1515_ntrev-2023-0176_(1).pdf 10.1515_ntrev-2023-0176_(1).pdf 1 DE4B7DC1A40347DAF878027D846ED805 a3c33e95cdca21042d24a7c4b586cadc3c06611401adea3b068390753fc6926c 729fa3fd-bf83-11ee-871e-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=175792 https://www.degruyter.com/document/doi/10.1515/ntrev-2023-0176/html 0 1290ca6d-bf84-11ee-871e-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=175794 Fakulteta za strojništvo 0 0 0