20.500.12556/DKUM-87111 Application of Lactobacillus reuteri B1/1 (Limosilactobacillus reuteri) improves immunological profile of the non-carcinogenic porcine-derived enterocytes In our previous studies, Lactobacillus reuteri B1/1, which was renamed Limosilactobacillus reuteri (L. reuteri), was able to modulate the production of pro-inflammatory cytokines and other components of the innate immune response in vitro and in vivo. In this study, we evaluated the effect of Lactobacillus reuteri B1/1 in two concentrations (1 x 10(7) and 1 x 10(9) CFU) on the metabolic activity, adherence ability and relative gene expression of pro-inflammatory interleukins (IL-1 beta, IL-6, IL-8, IL-18), lumican and olfactomedin 4 produced by non-carcinogenic porcine-derived enterocytes (CLAB). CLAB cells were cultured in a 12-well cell culture plate at a concentration of 4 x 10(5) cells/well in DMEM medium in a controlled humidified atmosphere for 48 h. A 1 mL volume of each probiotic bacterial suspension was added to the CLAB cells. Plates were incubated for 2 h and 4 h. Our results revealed that L. reuteri B1/1 was able to adhere to CLAB cells in sufficient numbers in both concentrations. In particular, the concentration of 109 L. reuteri B1/1 allowed to modulate the gene expression of pro-inflammatory cytokines, as well as to increase the metabolic activity of the cells. In addition, administration of L. reuteri B1/1 in both concentrations significantly stimulated gene expression for both proteins in the CLAB cell line after 4 h of incubation. Lactobacillus reuteri B1/1 CLAB cells pro-inflammatory cytokine metabolic activity celice citokini presnovne aktivnosti true false true MDPI Angleški jezik Slovenski jezik Znanstveno delo 2024-02-20 08:09:38 2024-02-20 08:44:57 2024-02-21 03:09:32 0000-00-00 00:00:00 2023 0 0 Str. 1-14 Št. 5, št. članka 1090 Letn. 13 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2023-03-22 2023-04-25 2023-04-27 579 150715651 10.3390/life13051090 2075-1729 Karaffova-2023-Application_of__i_Lactobacillus.pdf Karaffova-2023-Application_of__i_Lactobacillus.pdf 1 97E67CA8B1C9FBFE0A28C4F8C1965013 af699bb3d9e610eafd343bddcea4cb8d5c5910543a44b0faf7e76b2cbfcd47e6 476cf04d-cfc3-11ee-b227-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=176299 https://doi.org/10.3390/life13051090 0 03ab635c-cfbf-11ee-b227-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=176298 Fakulteta za kmetijstvo in biosistemske vede 0 0 0