20.500.12556/DKUM-87128 UCHL1, besides leptin and fibronectin, also could be a sensitive marker of the relapsing–remitting type of multiple sclerosis Research on the markers of immunoregulatory response in multiple sclerosis (MS) is still of great importance. The aim of our study was the evaluation of leptin, fibronectin, and UCHL1 concentrations as potential biomarkers of a relapsing-remitting type of MS (RRMS). Surface Plasmon Resonance Imaging (SPRI) biosensors were used for the evaluation of proteins concentrations in 100 RRMS patients and 46 healthy volunteers. Plasma leptin, fibronectin, and UCHL1 concentrations were significantly higher in RRMS patients compared to the control group (p < 0.001, respectively). UCHL1 concentration evaluation revealed the highest diagnostic sensitivity (100%) and negative predictive value (100%) in differentiating MS patients from healthy individuals. There was no significant difference in the UCHL1 concentrations depending on the patient's sex, the presence of relapse within the last 24 months, and the EDSS value (p > 0.05, respectively). In RRMS patients UCHL1 concentration positively correlated with fibronectin levels (r = 0.3928; p < 0.001). In the current cohort of patients plasma UCHL1 concentration was independent of the time of MS relapse and the severity of neurological symptoms. Thus current study may indicate that plasma UCHL1, besides leptin and fibronectin, also could be a promising high-sensitive potential biomarker of relapsing-remitting type of MS. However, these results should be validated with a larger group of patients, taking into account neuroimaging and cerebrospinal fluid analysis data, and by comparing them to patients with other neurological diseases as a control group. immunoregulatory response multiple sclerosis biomarkers leptin fibronectins ubiquitin thiolesterase imunoregulacijski odziv multipla skleroza biomarkerji leptin fibronektini ubikvitin tioesteraza true false true Nature Publishing Group Angleški jezik Slovenski jezik Znanstveno delo 2024-02-21 13:49:51 2024-02-21 15:00:11 2024-02-22 03:10:28 0000-00-00 00:00:00 2023 0 0 Str. 1-10 Št. članka 3423 Letn. 13 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-11-08 2023-02-20 2023-02-28 616.8 143814659 10.1038/s41598-023-30237-3 2045-2322 Górska-2023-UCHL1,_besides_leptin_and_fibronec.pdf Górska-2023-UCHL1,_besides_leptin_and_fibronec.pdf 1 64688FD3399558A6896539A5DEB73039 38adac2b65b958f6fcdabf3e090677814ad37c75738fd9c7c25f4289715a0ea9 3d2cb41f-d0c1-11ee-bf60-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=176345 https://doi.org/10.1038/s41598-023-30237-3 1 b58031f5-d0b7-11ee-bf60-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=176340 Medicinska fakulteta 0 0 0