20.500.12556/DKUM-87390 The development of an electropolymerized, molecularly imprinted polymer (MIP) sensor for insulin determination using single-drop analysis An electrochemical sensor for the detection of insulin in a single drop (50 μL) was developed based on the concept of molecularly imprinted polymers (MIP). The synthetic MIP receptors were assembled on a screen-printed carbon electrode (SPCE) by the electropolymerization of pyrrole (Py) in the presence of insulin (the protein template) using cyclic voltammetry. After electropolymerization, insulin was removed from the formed polypyrrole (Ppy) matrix to create imprinting cavities for the subsequent analysis of the insulin analyte in test samples. The surface characterization, before and after each electrosynthesis step of the MIP sensors, was performed using atomic force microscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The performance of the developed MIP–SPCE sensor was evaluated using a single drop of solution containing K3Fe(CN)6 and the square-wave voltammetry technique. The MIP–SPCE showed a linear concentration range of 20.0–70.0 pM (R2 = 0.9991), a limit of detection of 1.9 pM, and a limit of quantification of 6.2 pM. The rapid response time to the protein target and the portability of the developed sensor, which is considered a disposable MIP-based system, make this MIP–SPCE sensor a promising candidate for point-of-care applications. In addition, the MIP–SPCE sensor was successfully used to detect insulin in a pharmaceutical sample. The sensor was deemed to be accurate (the average recovery was 108.46%) and precise (the relative standard deviation was 7.23%). electrochemical sensors electropolymerisation electron microscopy elektrokemijski senzorji elektropolimerizacija mikroskopija true false true Royal Society of Chemistry Angleški jezik Slovenski jezik Članek v reviji 2024-03-14 14:45:00 2024-03-14 15:27:50 2024-04-16 10:24:36 0000-00-00 00:00:00 2023 0 0 Str. 1102-1115 Št. 5 Letn. 148 First published 24 Jan 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-12-12 2023-01-20 2023-01-24 544.5/.6 140143875 10.1039/D2AN02025D 1364-5528 Zidaric-2023-The_development_of_an_electropoly.pdf Zidaric-2023-The_development_of_an_electropoly.pdf 1 BD153EF8A063AC26887ED80FEC3C38C7 9bd72aed0ed4f9137e259f456fd994d3c6cc738886feaeb85aa7aaf077e1f49e c237b4b0-e20e-11ee-8231-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=176890 DOI https://doi.org/10.1039/D2AN02025D 0 dce09e61-e20e-11ee-8231-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=176891 Fakulteta za kemijo in kemijsko tehnologijo Medicinska fakulteta 0 0 0