20.500.12556/DKUM-88054 Bacterial nanocellulose loaded with bromelain and nisin as a promising bioactive material for wound debridement The bacterial nanocellulose (BnC) membranes were produced extracellularly by a novel aerobic acetic acid bacterium Komagataeibacter melomenusus. The BnC was modified in situ by adding carboxymethyl cellulose (CMC) into the culture media, obtaining a BnC-CMC product with denser fibril arrangement, improved rehydration ratio and elasticity in comparison to BnC. The proteolytic enzyme bromelain (Br) and antimicrobial peptide nisin (N) were immobilized to BnC matrix by ex situ covalent binding and/or adsorption. The optimal Br immobilization conditions towards the maximized specific proteolytic activity were investigated by response surface methodology as factor variables. At optimal conditions, i.e., 8.8 mg/mL CMC and 10 mg/mL Br, hyperactivation of the enzyme was achieved, leading to the specific proteolytic activity of 2.3 U/mg and immobilization efficiency of 39.1 %. The antimicrobial activity was observed against Gram-positive bacteria (S. epidermidis, S. aureus and E. faecalis) for membranes with immobilized N and was superior when in situ modified BnC membranes were used. N immobilized on the BnC or BnC-CMC membranes was cytocompatible and did not cause changes in normal human dermal fibroblast cell morphology. BnC membranes perform as an efficient carrier for Br or N immobilization, holding promise in wound debridement and providing antimicrobial action against Gram-positive bacteria, respectively. bacterial nanocellulose bromelain CMC debridement antimicrobial bakterijska nanoceluloza protimikrobna sredstva true false true Elsevier Angleški jezik Slovenski jezik https://www.sciencedirect.com/journal/international-journal-of-biological-macromolecules Članek v reviji 2024-04-09 03:43:59 2026-04-01 13:48:45 2026-04-02 03:19:03 0000-00-00 00:00:00 2024 0 0 20 str. part 2, [article no.] 131329 Vol. 266 May 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-01-14 2024-03-31 2024-04-03 577:543.2/.9 191447299 10.1016/j.ijbiomac.2024.131329 1879-0003 1-s2.0-S0141813024021342-main.pdf 1-s2.0-S0141813024021342-main.pdf 1 F9B481C60339CCF890B3A1AC7D9B0BB2 b71acc76eb070012d8a95e5e6fa428dc63e0b19625dd9da32d6cc69e3c1751ad a5c9a66b-2dc0-11f1-b2eb-00155d0001a1 https://dk.um.si/Dokument.php?lang=slv&id=204970 https://www.sciencedirect.com/science/article/pii/S0141813024021342?via%3Dihub 0 4f5d1f79-2dc0-11f1-b2eb-00155d0001a1 https://dk.um.si/Dokument.php?lang=slv&id=204968 Fakulteta za strojništvo 0 0 0