20.500.12556/DKUM-88194 Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus ▫$AV436^T$▫ and Komagataeibacter xylinus LMG 1518 The microbial production of cellulose using different bacterial species has been extensively examined for various industrial applications. However, the cost-effectiveness of all these biotechnological processes is strongly related to the culture medium for bacterial cellulose (BC) production. Herein, we examined a simple and modified procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium for BC production by acetic acid bacteria (AAB). The central composite design (CCD) was used to optimise the GP hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents (4.8 g/L). The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/ L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane). The membranes were synthesized in only 4 days of bacteria culturing, 1 st day with shaking, followed by 3 days of static incubation. The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile modulus, and elongation, respectively. Here presented study is the first report on utilising a GP-hydrolysate without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste material. The scale-up protocol of the scheme presented here will be needed for the cost-optimisation of BC production at the industrial levels. grape pomace hydrolysate bacterial cellulose acetic acid bacteria Komagataeibacter melomenusus true true true Elsevier Angleški jezik Angleški jezik Članek v reviji 2024-04-11 03:53:43 2024-07-26 10:49:31 2024-08-08 08:53:15 0000-00-00 00:00:00 2023 0 0 16 str. [article no.] ǂ125368 Vol. 244 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2023-04-16 2023-06-11 2023-06-15 579.6 155994883 10.1016/j.ijbiomac.2023.125368 0141-8130 RAZ_Gorgieva_Selestina_2023.pdf RAZ_Gorgieva_Selestina_2023.pdf 1 2C73066B287F241767D911392EE6C56E c888d2714297613ac8ff941ccf35f8890ac5ea784dc4ed56876e22c377816578 a818ea26-4b2a-11ef-8d59-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=181809 https://doi.org/10.1016/j.ijbiomac.2023.125368 0 c4d296f7-4b2a-11ef-8d59-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=181810 Fakulteta za strojništvo Fakulteta za naravoslovje in matematiko 0 0 0