20.500.12556/DKUM-88322 Bioactive functional nanolayers of chitosan-lysine surfactant with single- and mixed-protein-repellent and antibiofilm properties for medical implants Medical implant-associated infections resulting from biofilm formation triggered by unspecific protein adsorption arethe prevailing cause of implant failure. However, implant surfaces rendered with multifunctional bioactive nanocoatings offer apromising alternative to prevent the initial attachment of bacteria and effectively interrupt biofilm formation. The need to researchand develop novel and stable bioactive nanocoatings for medical implants and a comprehensive understanding of their properties incontact with the complex biological environment are crucial. In this study, we developed an aqueous stable and crosslinker-freepolyelectrolyte−surfactant complex (PESC) composed of a renewable cationic polysaccharide, chitosan, a lysine-based anionicsurfactant (77KS), and an amphoteric antibiotic, amoxicillin, which is widely used to treat a number of infections caused by bacteria.We successfully introduced the PESC as bioactive functional nanolayers on the“model”and“real”polydimethylsiloxane (PDMS)surfaces under dynamic and ambient conditions. Besides their high stability and improved wettability, these uniformly depositednanolayers (thickness: 44−61 nm) with mixed charges exhibited strong repulsion toward three model blood proteins (serumalbumin,fibrinogen, andγ-globulin) and their competitive interactions in the mixture in real-time, as demonstrated using a quartzcrystal microbalance with dissipation (QCM-D). The functional nanolayers with a maximum negative zeta potential (ζ:−19 to−30mV at pH 7.4), water content (1628−1810 ng cm−2), and hydration (low viscosity and elastic shear modulus) correlated with themass, conformation, and interaction nature of proteins. In vitro antimicrobial activity testing under dynamic conditions showed thatthe charged nanolayers actively inhibited the growth of both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcusaureus) bacteria compared to unmodified PDMS. Given the ease of fabrication of multifunctional and charged biobased coatingswith simultaneous protein-repellent and antimicrobial activities, the limitations of individual approaches could be overcome leadingto a better and advanced design of various medical devices (e.g., catheters, prosthetics, and stents). silicone implants protein-repellent antimicrobial chitosan lysine bioactive coatings adsorption QCM-D silikonski vsadki biomedicinski materiali protimikrobnost hitozan bioaktivni premazi adsorpcija true false true ACS Publications Angleški jezik Slovenski jezik https://pubs.acs.org/doi/10.1021/acsami.1c01993# Neznano 2024-04-15 16:50:11 2024-04-15 16:50:12 2024-04-16 03:53:26 0000-00-00 00:00:00 2021 0 0 str. 23352–23368 iss. 20 Vol. 13 May 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2021-01-29 2021-05-04 2021-05-17 677.017:579 63734787 10.1021/acsami.1c01993 1944-8244 ajdnik-et-al-2021-bioactive-functional-nanolayers-of-chitosan-lysine-surfactant-with-single-and-mixed-protein-repellent.pdf ajdnik-et-al-2021-bioactive-functional-nanolayers-of-chitosan-lysine-surfactant-with-single-and-mixed-protein-repellent.pdf 1 AB33C47B2993FC98239EDF17508702AC b39e71afac8d5628419b726e8d6ecc4f6cae1100a5b2decc86051303bd2913b5 30478e0c-fb38-11ee-8f8b-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=178588 https://pubs.acs.org/doi/10.1021/acsami.1c01993# 0 4abf9e03-fb38-11ee-8f8b-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=178589 Fakulteta za strojništvo 0 0 0