20.500.12556/DKUM-88340 The immobilization of β-galactosidase on glass fiber rolls The usability of glass fibers as immobilization support with a porous open structure was investigated. We developed a method to immobilize the enzyme β-galactosidase on special glass fiber rolls. The new method is simple, non-expensive and industrially applicable. Glutaraldehyde was used as a non-specific cross-linking agent for the covalent binding of β-galactosidase on modified glass fibers. The efficiency of immobilization was tested with the known hydrolysis of lactose. All experiments were performed in a continuous laboratory reactor. The influence of the reaction temperature (20, 25 and 30 °C), the substrate flow rate (1, 2 and 3 mL/min) and the pH of the reaction medium (6, 7 and 8) on the conversion was investigated. The reaction efficiency was monitored by measuring the glucose concentration with a spectrophotometer. High immobilization efficiency, enzyme activity and stability were obtained. The optimal reaction temperature, substrate flow rate and pH were found. The activity and stability of the enzyme entrapped on the glass fiber rolls remained almost unchanged during reuse, which is promising for potential industrial applications. beta-galaktosidase immobilization glass fiber lactose beta galaktozidaza imobilizacija steklena vlakna true false true MDPI Angleški jezik Slovenski jezik https://www.mdpi.com/journal/catalysts Članek v reviji 2024-04-17 10:09:25 2024-04-17 10:09:29 2024-04-18 03:12:23 0000-00-00 00:00:00 2023 0 0 11 str. no. 12, [article no.] 1503 Vol. 13 5 Dec. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2023-11-16 2023-12-07 2023-12-09 66.02 176801027 10.3390/catal13121503 2073-4344 catalysts-13-01503.pdf catalysts-13-01503.pdf 1 DD0758120CB309B53831E83924CBB010 1ee3b87b16100ffaee1c7b1d72eda7fb0136e4b4b18dc9ae7a1047d4c228d620 9cd5acd2-fc92-11ee-a244-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=178657 https://www.mdpi.com/2073-4344/13/12/1503 0 b51d4f26-fc92-11ee-a244-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=178658 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0