20.500.12556/DKUM-88536 Antimicrobial properties of different hop (Humulus lupulus) genotypes The antimicrobial activity of hop extracts obtained from different hop genotypes were investigated against Staphylococcus aureus and Lactobacillus acidophilus. In this study the pure xanthohumol, purified β-acids rich fraction, as well as α-acids with β-acids rich fraction were used to test antimicrobial activity against Staphylococcus aureus and Lactobacillus acidophilus; whereby, the antimicrobial activity of different hop extracts against Lactobacillus acidophilus was studied for the first time. Microbial susceptibility to purified hydroacetonic extracts from different hop varieties was investigated by the broth microdilution assay to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The hop hydroacetonic extracts were more effective against Staphylococcus aureus than against Lactobacillus acidophilus. Strong inverse correlations of MIC and MBC values were obtained with xanthohumol, cohumulone, n+adhumulone, colupulone and n+adlupulone contents, suggesting that the identified chemical hop compounds are directly responsible for antimicrobial effects. Moreover, the effect of the growth medium strength on the MIC values of hop extracts against Staphylococcus aureus was systematically investigated for the first time. The current study also reveals the effect of different hop extracts on Staphylococcus aureus, which responds to their presence by lag phase extension and generation time prolongation. hop extract xanthohumol Staphylococcus aureus Lactobacillus acidophilus minimum inhibitory concentration minimum bactericidal concentration true false true MDPI Angleški jezik Angleški jezik https://www.mdpi.com/journal/plants Članek v reviji 2024-05-09 13:34:22 2024-05-09 13:34:24 2024-05-10 03:24:23 0000-00-00 00:00:00 2023 0 0 str. 1-19 art. 120 iss. 1 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-11-24 2022-12-20 2023-12-26 633.791 135406339 10.3390/plants12010120 2223-7747 plants-12-00120.pdf plants-12-00120.pdf 1 3EAA2C3E7036AFF8287B83EFF5DA3EC6 2711117f387a4fc0308705bd041f307bb20790f86c5f320bf8d30ccdbbd8a2b2 c5aa56bf-0df8-11ef-b0c0-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=179162 https://www.mdpi.com/2223-7747/12/1/120 1 19b28276-0df8-11ef-b0c0-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=179159 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0