20.500.12556/DKUM-88632 Genetic diversity of exopolysaccharides from acetic acid bacteria isolates originating from apple cider vinegars Acetic acid bacteria (AAB) produce acetic acid but are also gaining importance as safe microorganisms for producing extracellular polysaccharides (EPSs). The best-known homopolysaccharides among them are cellulose and levan. In addition, acetic acid bacteria also produce heteropolysaccharides, water-soluble acetans. Isolates from the broth of organic and conventional apple cider vinegar production were screened for biofilm production. Phenotypic and genomic diversity of EPS-producing isolates was assessed. The diversity of phenotypically different EPSs of apple cider vinegar isolates was investigated at the gene level for the following novel strains: Komagataeibacter (K.) melomenusus SI3083, K. oboediens SI3053, K. pomaceti SI3133, and Gluconacetobacter (Ga.) entanii SI2084. Strain K. melomenusus SI3083 possesses cellulose operons bcs1, bcs2, and bcs4 together with the type I acetan cluster in the absence of the levan operon, strain K. oboediens SI3053 has the operons bcs1, bcs2, bcs3, and bcs4, the levan operon, and the acetan cluster (type I), and the strains K. pomaceti SI3133 and Ga. entanii SI2084 both contain recently described novel ace-type II cluster in addition to the incomplete operon bcs1. A comparison of the genetic diversity of these EPSs to those of the reference strains suggests that the studied EPSs are not species-descriptive. The results of this study deepen our understanding of the genetic variability of the EPS genes in AAB, thereby enabling us to better characterize and exploit the various insoluble and soluble exopolysaccharides produced by AAB for biotechnological applications in the future. acetic acid bacteria genomes apple cider vinegar microbiota biofilm production bacterial cellulose acetan Acetobacter Komagataeibacter genomi ocetnokislinskih bakterij mikrobiota jabolčnega kisa proizvodnja biofilma bakterijska celuloza acetan Acetobacter Komagataeibacter true true true German Multidisciplinary Publishing Center Angleški jezik Slovenski jezik Članek v reviji 2024-05-17 03:18:41 2024-09-26 15:18:19 2024-11-22 13:46:48 0000-00-00 00:00:00 2022 0 0 str. 1-18 iss. 1 Vol. 2 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 2022-02-04 2022-02-26 579 100508675 10.51585/gjm.2022.1.0011 2749-0149 RAZ_Vajdic_Tadeja_2022.pdf RAZ_Vajdic_Tadeja_2022.pdf 1 68FEB36C942FB65E5B086E7BA2062A96 4f790b39c72b14aad1dda0787f4fdb962e6e66ad7a7a8765c49fe8b7d4c96895 3377e97f-7c09-11ef-8e3e-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=185944 https://doi.org/10. 51585/gjm.2022.1.0011 0 6a2c8d9a-7c09-11ef-8e3e-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=185945 Fakulteta za naravoslovje in matematiko 0 0 0