20.500.12556/DKUM-88634 Glucose-stimulated calcium dynamics in beta cells from male C57BL/6J, C57BL/6N, and NMRI mice a comparison of activation, activity, and deactivation properties in tissue slices Although mice are a very instrumental model in islet beta cell research, possible phenotypic differences between strains and substrains are largely neglected in the scientific community. In this study, we show important phenotypic differences in beta cell responses to glucose between C57BL/6J, C57BL/6N, and NMRI mice, i.e., the three most commonly used strains. High-resolution multicellular confocal imaging of beta cells in acute pancreas tissue slices was used to measure and quantitatively compare the calcium dynamics in response to a wide range of glucose concentrations. Strain- and substrain-specific features were found in all three phases of beta cell responses to glucose: a shift in the dose-response curve characterizing the delay to activation and deactivation in response to stimulus onset and termination, respectively, and distinct concentration-encoding principles during the plateau phase in terms of frequency, duration, and active time changes with increasing glucose concentrations. Our results underline the significance of carefully choosing and reporting the strain to enable comparison and increase reproducibility, emphasize the importance of analyzing a number of different beta cell physiological parameters characterizing the response to glucose, and provide a valuable standard for future studies on beta cell calcium dynamics in health and disease in tissue slices. beta cell mouse models calcium imaging glucose-dependence tissue slice beta celice mišji modeli kalcijevo slikanje glukozna odvisnost tkivne rezine true false true Frontiers Research Foundation Angleški jezik Slovenski jezik Znanstveno delo 2024-05-17 03:19:05 2024-07-15 14:55:49 2024-10-15 14:10:46 0000-00-00 00:00:00 2022 0 0 Str. 1-16 št. članka 867663 Letn. 13 March 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-02-04 2022-02-23 2022-03-24 612.349.7 103005187 10.3389/fendo.2022.867663 1664-2392 Pohorec-2022-Glucose-Stimulated_Calcium_Dynami.pdf Pohorec-2022-Glucose-Stimulated_Calcium_Dynami.pdf 1 33BA39396E86135AD44159AE65194A16 39a393e805d25249f2c0ebcaddc32838b35ee0ae57205bc2c04b98961b85e269 66ea0d5c-42a9-11ef-99b3-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=181495 https://doi.org/10.3389/fendo.2022.867663 0 7512a90b-13eb-11ef-88d0-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=179373 Medicinska fakulteta Fakulteta za naravoslovje in matematiko 0 0 0