20.500.12556/DKUM-88637 Diversity and content of carotenoids and other pigments in the transition from the green to the red stage of Haematococcus pluvialis microalgae identified by HPLC-DAD and LC-QTOF-MS H. pluvialis is a unicellular freshwater alga containing many bioactive compounds, especially carotenoids, which are the strongest antioxidants among the pigments. This study evaluates the composition and content of carotenoids and other pigments in both stages of algae life cycle, especially in the green vegetative stage, less studied in comparison to the red stage. To determine the composition and content of carotenoids, a combination of HPLC-DAD and LC-QTOF-MS was used. The content of carotenoids in the green vegetative stage was significantly lower than in the red vegetative stage. In the green vegetative stage, 16 different carotenoids and other pigments were identified. Among the total 8.86 mg g−1 DW of pigments, 5.24 mg g−1 DW or 59% of them were chlorophyll a with its derivatives, and 3.62 mg g−1 DW or 41% of them were free carotenoids. After the transition from the green to the red stage, the carotenoid composition was replaced by secondary carotenoids, astaxanthin and its esters, which predominated in the whole carotenoid composition. In addition to free astaxanthin, 12 astaxanthin monoesters, 6 diesters and 13 other carotenoids were determined. The majority of 37.86 mg g−1 DW pigments were monoesters. They represented 82% of all pigments, and their content was about 5 times higher than both, diesters (5.91 mg g−1 DW or 12% of all) and free carotenoids (2.4 mg g−1 DW or 6% of all). The results of the study contribute to the data on the overall pigment composition and content of H. pluvialis algae and provide the basis for further improvement of cultivation of the H. pluvialis algae. antioxidants astaxanthin chlorophylls bioactive compounds algae Haematococcus life cycle pigment composition secondary carotenoids true true true MDPI Angleški jezik Angleški jezik Članek v reviji 2024-05-17 03:19:35 2024-05-21 09:48:07 2024-05-29 08:24:20 0000-00-00 00:00:00 2022 0 0 14 str. iss. 8 Vol. 11 Apr. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-03-15 2022-04-07 2022-04-09 582.3 104399875 10.3390/plants11081026 2223-7747 RAZ_Grujic_Jasa_Veno_2022.pdf RAZ_Grujic_Jasa_Veno_2022.pdf 1 1B553D913D58A379FCCB00BFADF18958 a2e9586bad0bc5c7f5d3d93bffbd1e2cf39b73cdd56834bb139f2d7912df4972 c3aae016-1746-11ef-84d7-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=179540 https://www.mdpi.com/2223-7747/11/8/1026 0 ab5c36d6-1746-11ef-84d7-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=179539 Fakulteta za naravoslovje in matematiko Pedagoška fakulteta Fakulteta za kmetijstvo in biosistemske vede 0 0 0