20.500.12556/DKUM-88722 Slow growth storage of Berula erecta in vitro - effect of sucrose, sorbitol and temperature Počasna rast ozkolistnega koščca (Berula erecta) in vitro - vpliv saharoze, sorbitola in temperature It has been established that in vitro storage of Berula erecta (Huds.) Coville by slow growth would rationalize the culture maintenance by reducing the number of transfers to fresh medium. Attempts of slow growth were made while maintaining viability by adding sugars and sugar alcohols to the MS medium and lowering the culture temperature in the growth chambers. The effect of low temperatures (13°C and 4°C), different combinations and concentrations of sorbitol (20, 40 g L-1) and sucrose (30, 40 g L-1), and light was tested to determine the viability and ability of B. erecta to recover growth. Increasing sucrose concentration did not inhibit or slow growth. At 23 ± 2°C, the addition of 20 g L-1 sorbitol successfully inhibited growth, while the addition of 40 g L-1 sorbitol resulted in plant senescence after two months of culturing. Storage of B. erecta at 13°C did not effectively inhibit growth and recovery ability in any treatment. The most effective way to maintain B. erecta for long-term slow growth storage in tissue culture while retaining a high level of viability with minimal growth was to culture the plants at 4°C in the dark. This kept the plants viable and capable of regeneration for several months. In vitro hramba ozkolistnega koščca (Berula erecta (Huds.) Coville)) s počasno rastjo bi racionalizirala vzdrževanje tkivne kulture, saj bi zmanjšala število prestavljanj na sveže gojišče. Počasno rast ob hkratnem ohranjanju viabilnost smo poskušali doseči z dodajanjem sladkorjev in sladkornih alkoholov v MS gojišče in nižanjem temperature v rastnih komorah. Preizkusili smo vpliv nizkih temperatur (13 °C in 4 °C), različnih kombinacij in koncentracij sorbitola (20, 40 g L-1) in saharoze (30, 40 g L-1) ter svetlobe na viabilnost in sposobnost povrnitve rasti B. erecta. Povišanje koncentracije saharoze ni zavrlo rasti. Pri 23 ± 2 °C je dodatek 20 g L-1 sorbitola uspešno zavrl rast, dodatek 40 g L-1 sorbitola je po dveh mesecih kulture vodil v senescenco rastlin. Hramba B. erecta pri 13 °C v nobeni obravnavi ni učinkovito zavrla rasti. Kot najbolj učinkovit način za dolgoročno hrambo B. erecta s počasno rastjo se je izkazala hramba rastlin pri 4 °C v temi. Ob minimalni stopnji rasti je B. erecta ohranila visoko stopnjo viabilnosti, pri čemer so rastline ostale več mesecev nespremenjeno viabilne ter sposobne ponovne regeneracije. slow growth storage viability sucrose sorbitol temperature Berula erecta hramba s počasno rastjo viabilnost saharoza sorbitol temperatura Berula erecta true true true University of Maribor, University Press Angleški jezik Slovenski jezik Članek v reviji 2024-05-22 03:28:45 2024-05-23 13:03:24 2024-05-24 04:00:15 0000-00-00 00:00:00 2022 0 0 str. 33-41 no. 1 Vol. 19 jun. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-05-22 581.5 118238211 10.18690/agricultura.19.1.33-41.2022 1580-8432 https://journals.um.si/index.php/agricultura/article/view/2190 1 a42790e6-17da-11ef-8ccf-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=179585 RAZ_Ambrozic-Dolinsek_Jana_2022.pdf RAZ_Ambrozic-Dolinsek_Jana_2022.pdf 1 D89C326C809D00B164AF29E2C9393D59 aa14486fe3a71b2c8e6b685b41cc821e5d5d9847a83d845db0b6d3945606e368 41c7ea00-18ef-11ef-af6e-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=179668 Fakulteta za naravoslovje in matematiko Pedagoška fakulteta 0 0 0