20.500.12556/DKUM-88723 Use of emulsion-templated, highly porous polyelectrolytes for in vitro germination of chickpea embryos a new substrate for soilless cultivation The application of highly porous and 3D interconnected microcellular polyelectrolyte polyHIPE (PE-PH) monoliths based on (3-acrylamidopropyl)-trimethylammonium chloride as soilless cultivation substrates for in vitro embryo culture is discussed. The embryo axes isolated from chickpea seeds are inoculated onto the surface of the monoliths and allowed to germinate. Germination study show that the newly disclosed PE-PH substrate performs much better than the conventionally used agar as the germination percentage, shoot and root length, fresh and dry weight as well as the number of leaves are enhanced. The PE-PHs exhibit a higher absorption capacity of the plant growth medium, that is, 36 g·g–1 compared to agar, that is, 20 g·g–1, and also survive autoclaving conditions without failing. The key advantage over standard agar substrates is that they can be reused several times and also without prior sterilization. These results suggest that PE-PHs with exceptional absorption/retention properties and robustness have great potential as soilless substrates for in vitro plant cultivation. absorption catalyst supports germination plants porosity absorpcija katalizatorski nosilci kaljivost rastline poroznost true false true American Chemical Society Angleški jezik Slovenski jezik Znanstveno delo 2024-05-22 03:29:06 2024-07-15 12:03:50 2024-07-16 03:57:49 0000-00-00 00:00:00 2022 0 0 Str. 3452-3457 Št. 8 Letn. 23 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-05-11 2022-06-21 2022-07-08 577.2 118269187 10.1021/acs.biomac.2c00593 1525-7797 Kovacic-2022-Use_of_Emulsion-Templated,_Highly.pdf Kovacic-2022-Use_of_Emulsion-Templated,_Highly.pdf 1 63C45CD6873FBB42DD02F668E7CBA680 93873914d2e58644b09b5823dd6f873348c4c9302f96d2da7e99c529d8972703 1c4e8c09-4291-11ef-99b3-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=181473 https://doi.org/10.1021/acs.biomac.2c00593 0 525545d1-4291-11ef-99b3-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=181474 Fakulteta za naravoslovje in matematiko 0 0 0