20.500.12556/DKUM-89127 Children gut microbiota exhibits a different composition and metabolic profile after in vitro exposure to Clostridioides difficile and increases its sporulation Clostridioides difficile (Clostridium difficile) infection (CDI) is one of the main public health concerns in adults, while children under 2 years of age are often colonized asymptomatically. In both adults and children, CDI is strongly associated with disturbances in gut microbiota. In this study, an in-vitro model of children gut microbiota was challenged with vegetative cells or a conditioned media of six different toxigenic C. difficile strains belonging to the ribotypes 027, 078, and 176. In the presence of C. difficile or conditioned medium the children gut microbiota diversity decreased and all main phyla (Bacteroidetes, Firmicutes, and Proteobacteria) were affected. The NMR metabolic spectra divided C. difficile exposed children gut microbiota into three clusters. The grouping correlated with nine metabolites (short chain fatty acids, ethanol, phenolic acids and tyramine). All strains were able to grow in the presence of children gut microbiota and showed a high sporulation rate of up to 57%. This high sporulation rate in combination with high asymptomatic carriage in children could contribute to the understanding of the reported role of children in C. difficile transmissions. biokemija črevesna mikrobiota črevesna flora presnova metabolizem otroci true false true Frontiers Research Foundation Angleški jezik Slovenski jezik Znanstveno delo 2024-06-17 11:58:36 2024-06-17 12:06:14 2024-06-18 03:23:25 0000-00-00 00:00:00 2022 0 0 Str. 1-11 Št. članka 1042526 Letn. 13 9. Dec. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-09-12 2022-11-17 2022-12-09 577 134179587 10.3389/fmicb.2022.1042526 1664-302X Horvat-2022-Children_gut_microbiota_exhibits_a.pdf Horvat-2022-Children_gut_microbiota_exhibits_a.pdf 1 D8D96E9D8E33E5BED6D39ECE3ECC7060 43609ad290d59387b700dd6e48999d4ac1881b8b47a3c4d78d499728d24cb800 1260fe27-2c91-11ef-be7d-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=180575 https://doi.org/10.3389/fmicb.2022.1042526 0 20cc7a6b-2c91-11ef-be7d-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=180576 Medicinska fakulteta 0 0 0