20.500.12556/DKUM-89251 Study of V[sub]2CT[sub]x-MXene Based Immunosensor for Sensitive Label-Free Impedimetric Detection of SARS-CoV-2 Spike Protein Rapid and reliable immunosensing is undoubtedly one of the priorities in the efficient management and combat against a pandemic, as society has experienced with the SARS-CoV-2 outbreak; simple and cost-effective sensing strategies are at the forefront of these efforts. In this regard, 2D-layered MXenes hold great potential for electrochemical biosensing due to their attractive physicochemical properties. Herein, we present a V$_2$CT$_x$ MXene-based sensing layer as an integral part of a label-free immunosensor for sensitive and selective detection of the SARS-CoV-2 spike protein. The sensor was fabricated on a supporting screen-printed carbon electrode using Nafion as an immobilizing agent for MXene and glutaraldehyde, the latter enabling effective binding of protein A for further site-oriented immobilization of anti-SARS-CoV-2 antibodies. A thorough structural analysis of the sensor architecture was carried out, and several key parameters affecting the fabrication and analytical performance of the immunosensor were investigated and optimized. The immunosensor showed excellent electroanalytical performance in combination with an impedimetric approach and exhibited a low detection limit of only 45 fM SARS-CoV-2 spike protein. Its practical applicability was successfully demonstrated by measuring the spike protein in a spiked artificial nasopharyngeal fluid sample. imunosenzorji polprevodniške nanostrukture proteini spike proteins immunosensors SARS-CoV-2 V2CTxMXene true false true ACS Publications (American Chemical Society) Angleški jezik Slovenski jezik https://pubs.acs.org/doi/epdf/10.1021/acsami.4c04567?ref=article_openPDF © 2024 The Authors Članek v reviji 2024-06-30 07:41:43 2024-08-29 08:10:43 2025-05-28 07:02:52 0000-00-00 00:00:00 2024 0 0 str. 30196-30208 iss. 23 Vol. 16 30 May 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-03-19 2024-05-15 2024-05-30 543.384 198606083 10.1021/acsami.4c04567 1944-8252 tasic-et-al-2024-study-of-v2ctx-mxene-based-immunosensor-for-sensitive-label-free-impedimetric-detection-of-sars-cov-2.pdf tasic-et-al-2024-study-of-v2ctx-mxene-based-immunosensor-for-sensitive-label-free-impedimetric-detection-of-sars-cov-2.pdf 1 4A0B567EC33D05B7E632D08CD32CB68E 6b81f6c4b03ab968fa69c085df2075974c80337a548a2c8bb30e74a4cbe16969 8a22a8ee-3a0e-11f0-80b9-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=191266 https://pubs.acs.org/doi/10.1021/acsami.4c04567 0 73ff6fd9-36a3-11ef-864a-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=180908 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0