20.500.12556/DKUM-89257 pH-dependence of glucose- dependent activity of beta cell networks in acute mouse pancreatic tissue slice Extracellular pH has the potential to affect various aspects of the pancreatic beta cell function. To explain this effect, a number of mechanisms was proposed involving both extracellular and intracellular targets and pathways. Here, we focus on reassessing the influence of extracellular pH on glucose-dependent beta cell activation and collective activity in physiological conditions. To this end we employed mouse pancreatic tissue slices to perform high-temporally resolved functional imaging of cytosolic Ca2+ oscillations. We investigated the effect of either physiological H+ excess or depletion on the activation properties as well as on the collective activity of beta cell in an islet. Our results indicate that lowered pH invokes activation of a subset of beta cells in substimulatory glucose concentrations, enhances the average activity of beta cells, and alters the beta cell network properties in an islet. The enhanced average activity of beta cells was determined indirectly utilizing cytosolic Ca2+ imaging, while direct measuring of insulin secretion confirmed that this enhanced activity is accompanied by a higher insulin release. Furthermore, reduced functional connectivity and higher functional segregation at lower pH, both signs of a reduced intercellular communication, do not necessary result in an impaired insulin release. insulin secretion membrane excitability potassium channels beta cell network collective activity calcium waves pancreatic islets pH-dependence izločanje inzulina razdražljivost membrane kalijevi kanali omrežje beta celic kolektivna dejavnost kalcijevi valovi true false true Frontiers Research Foundation Angleški jezik Slovenski jezik Znanstveno delo 2024-07-01 11:19:10 2024-07-01 12:09:09 2024-07-02 03:24:52 0000-00-00 00:00:00 2022 0 0 Str. 1-11 Vol. 13 Jun. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-04-09 2022-05-24 2022-06-28 616.8:577 117435907 10.3389/fendo.2022.916688 1664-2392 Postic-2022-pH-Dependence_of_Glucose-Dependent.pdf Postic-2022-pH-Dependence_of_Glucose-Dependent.pdf 1 0078D0BADC4C204D524439EE48E594B9 0315b71d30ba6ea8f1e17ebe705bd04a67dc55a1b0a076568f1ad91087ca3b46 b94e95f6-3791-11ef-836b-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=180932 https://doi.org/10.3389/fendo.2022.916688 0 c68bb26d-3791-11ef-836b-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=180933 Medicinska fakulteta Fakulteta za naravoslovje in matematiko 0 0 0